Bcl 10 (B Cell Leukemia 10, CIPER, mE10, CARMEN, CLAP)
Product Sizes
100ug
£748.00
B0807-12E-100UG
About this Product
- SKU:
- B0807-12E
- Application:
- Western Blot
- translate.label.attr.clone:
- 7H179
- Clonality:
- Monoclonal
- Extra Details:
- B cell CLL/leukemia 10 (Bcl-10), a 233 amino acid, 26.2kD protein, is the cellular homolog of the equine Herpes virus-2 gene (E10). Bcl-10 is also known as CARD like apoptotic protein (CLAP), CED-3/ICH-1 prodomain homologous E10-like regulator (CIPER), celluar-E10 (c-E10), and CARD-containing molecule enhancing NFkappaB (CARMEN). Bcl-10 contains a caspase recruitment domain (CARD) and has been shown to induce apoptosis and to activate NF-kappaB. Bcl-10 can interact with other CARD domain containing proteins including CARD9, 10, 11, and 14, which function as upstream regulators of NF-kappaB signaling. Defects in Bcl-10 may be involved in the pathogenesis of human malignancy. Applications: Suitable for use in Western Blot. Other applications not tested. Recommended Dilutions: Western Blot: 1ug/ml using Raji human Burkitt's lymphoma cell line, C2C12 mouse myoblast cell line, and L6 rat myoblast cell line. Optimal dilutions to be determined by the researcher. Storage and Stability: Lyophilized and reconstituted products are stable for 12 months after receipt at -20°C. Reconstitute with sterile PBS. Aliquot to avoid repeated freezing and thawing. Store at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
- Host:
- Mouse
- Immunogen:
- E. coli derived recombinant protein corresponding to Met1-Leu192 from human Bcl-10.
- Isotype:
- IgG1
- Physical State:
- Supplied as a lyophilized powder from PBS, 5% trehalose. Reconstitute with 200ul sterile PBS.
- Purity:
- Purified by Protein G affinity chromatography from hybridoma culture supernatant.
- Shipping Conditions:
- Blue Ice
- Specificity:
- Detects endogenous human, mouse, and rat Bcl-10 on Western Blots.
- Source:
- human
- Storage Conditions:
- -20[o]C
- Type:
- Antibodies:Monoclonal Antibody
