Bcl 2 (B Cell Leukemia 2) (HRP)
Product Sizes
100ul
£1055.00
B0807-05A-HRP-100UL
About this Product
- SKU:
- B0807-05A-HRP
- Application:
- Immunoprecipitation, Immunohistochemistry, Western Blot
- translate.label.attr.clone:
- 3G15 (Bcl-2–100)
- Clonality:
- Monoclonal
- Extra Details:
- Bcl-2 is a widely studied modulator of programmed cell death (apoptosis) in lymphoid cells. This 26kD integral membrane protein has been localized to several distinct subcellular locations including: the outer mitochondrial membrane, perinuclear membrane and the smooth endoplasmic reticulum. Overexpression of the Bcl-2 protein has been shown to prevent or delay many forms of programmed cell death induced by a variety of different stimuli including: growth factor deprivation, g-irradiation, glucocorticoids, and chemotherapeutic agents. The bcl-2 proto-oncogene was first identified at the breakpoint region of the t(14;18) chromosomal translocation found in a large percentage (85%) of human follicular B-cell lymphomas. This translocation results in transcriptional dysregulation of the bcl-2 gene and overexpression of the Bcl-2 protein. Originally, expression of the Bcl-2 protein was thought to be restricted to neoplastic cells in which the t(14;18) chromosomal translocation was present. However, subsequent studies demonstrated that Bcl-2 is in fact expressed in both normal T- and B-cells, as well as in a variety of lymphoproliferative disorders in which the t(14;18) translocation is not present. Expression of the Bcl-2 protein has been found to be regulated in a stage-specific manner during lymphoid development and is thought to be a survival signal for positive selection. However, the precise mechanism whereby Bcl-2 acts to inhibit programmed cell death remains to be elucidated. Applications: Suitable for use in ELISA, Western Blot (2,7), Immunocytochemistry, Immunoprecipitation and Immunohistochemistry. Other applications not tested. Recommended Dilutions: Immunohistochemistry: Frozen and paraffin sections Optimal dilutions to be determined by the researcher. Storage and Stability: Store product at 4°C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20°C. Aliquots are stable at -20°C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Note: Sodium azide is a potent inhibitor of peroxidase and should not be added to HRP conjugates. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Note: Applications are based on unconjugated antibody.
- Host:
- Mouse
- Immunogen:
- Synthetic peptide corresponding to residues 41-54 of the human Bcl-2 protein. This sequence is not shared by the mouse Bcl-2 protein.
- Isotype:
- IgG1,k
- Physical State:
- Supplied as a liquid in PBS, pH 7.2. No preservatives added. Labeled with Horseradish peroxidase (HRP).
- Purity:
- Purified by Protein A affinity chromatography.
- Shipping Conditions:
- Blue Ice
- Specificity:
- Recognizes human Bcl-2 protein and does not exhibit crossreactivity with other proteins. The Bcl-2 protein has been detected in human Jurkat cell lysate, human K562 cell lysate, human tonsil tissue.
- Source:
- human
- Storage Conditions:
- -20[o]C
- Supplier:
- United States Biological
- Type:
- Antibodies:Monoclonal Antibody
