Bcl 2 (B Cell Leukemia 2) (Biotin)
Product Sizes
100ul
£1055.00
B0807-05A-BIOTIN-100UL
About this Product
- SKU:
- B0807-05A-BIOTIN
- Application:
- Western Blot, Immunofluorescence, Immunohistochemistry, Flow Cytometry, Immunoprecipitation
- translate.label.attr.clone:
- 3G15 (Bcl-2–100)
- Clonality:
- Monoclonal
- Extra Details:
- Bcl-2 is a widely studied modulator of programmed cell death (apoptosis) in lymphoid cells. This 26kD integral membrane protein has been localized to several distinct subcellular locations including: the outer mitochondrial membrane, perinuclear membrane and the smooth endoplasmic reticulum. Overexpression of the Bcl-2 protein has been shown to prevent or delay many forms of programmed cell death induced by a variety of different stimuli including: growth factor deprivation, g-irradiation, glucocorticoids, and chemotherapeutic agents. The bcl-2 proto-oncogene was first identified at the breakpoint region of the t(14;18) chromosomal translocation found in a large percentage (85%) of human follicular B-cell lymphomas. This translocation results in transcriptional dysregulation of the bcl-2 gene and overexpression of the Bcl-2 protein. Originally, expression of the Bcl-2 protein was thought to be restricted to neoplastic cells in which the t(14;18) chromosomal translocation was present. However, subsequent studies demonstrated that Bcl-2 is in fact expressed in both normal T- and B-cells, as well as in a variety of lymphoproliferative disorders in which the t(14;18) translocation is not present. Expression of the Bcl-2 protein has been found to be regulated in a stage-specific manner during lymphoid development and is thought to be a survival signal for positive selection. However, the precise mechanism whereby Bcl-2 acts to inhibit programmed cell death remains to be elucidated. Applications: Suitable for use in ELISA, Flow Cytometry, Immunofluorescence, Immunocytochemistry, Western Blot, Immunoprecipitation and Immunohistochemistry. Other applications not tested. Recommended Dilutions: Immunohistochemistry: Frozen and paraffin sections Optimal dilutions to be determined by the researcher. Storage and Stability: Store product at 4°C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20°C. Aliquots are stable at -20°C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Note: Applications are based on unconjugated antibody.
- Host:
- Mouse
- Immunogen:
- Synthetic peptide corresponding to residues 41-54 of the human Bcl-2 protein. This sequence is not shared by the mouse Bcl-2 protein.
- Isotype:
- IgG1,k
- Physical State:
- Supplied as a liquid in PBS, pH 7.2. No preservatives added. Labeled with Biotin.
- Purity:
- Purified by Protein A affinity chromatography.
- Shipping Conditions:
- Blue Ice
- Specificity:
- Recognizes human Bcl-2 protein and does not exhibit crossreactivity with other proteins. The Bcl-2 protein has been detected in human Jurkat cell lysate, human K562 cell lysate, human tonsil tissue.
- Source:
- human
- Storage Conditions:
- -20[o]C
- Supplier:
- United States Biological
- Type:
- Antibodies:Monoclonal Antibody
