BARD1 (BRCA1 Associated Ring Domain 1)
Product Sizes
50ug
£448.00
B0085-01C-50UG
About this Product
- SKU:
- B0085-01C
- Application:
- Immunoprecipitation, Western Blot
- translate.label.attr.clone:
- 8C29
- Clonality:
- Monoclonal
- Extra Details:
- BARD1 interacts with the N-terminal region of BRCA1. In addition to its ability to bind BRCA1 in vivo and in vitro, BARD1 shares homology with the 2 most conserved regions of BRCA1: the N-terminal ring motif and the C-terminal BRCT domain. The ring motif is a cysteine-rich sequence found in a variety of proteins that regulate cell growth, including the products of tumor suppressor genes and dominant protooncogenes. The BARD1 protein also contains 3 tandem ankyrin repeats. The BARD1/BRCA1 interation is disrupted by tumorigenic amino acid substitutions in BRCA1, implying that the formation of a stable complex between these proteins may be an essential aspect of BRCA1 tumor suppression. BARD1 may be the target of oncogenic mutations in breast or ovarian cancer. Applications: Suitable for use in Dot Blot, Western Blot and Immunoprecipitation. Other applications have not been tested. Recommended Dilution: Western Blot: 0.2-2ug/ml. Immunoprecipitation: 100-500ug/sample Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
- Host:
- Mouse
- Immunogen:
- Synthetic peptide corresponding to aa50-200 from the internal region of human BARD1.
- Isotype:
- IgG1
- Physical State:
- Supplied as a liquid in PBS, pH 7.4, 1% BSA, 0.05% sodium azide.
- Purity:
- Purified by Protein G affinity chromatography from hybridoma culture supernatant
- Shipping Conditions:
- Blue Ice
- Specificity:
- Recognizes human BARD1.
- Source:
- human
- Storage Conditions:
- -20[o]C
- Supplier:
- United States Biological
- Type:
- Antibodies:Monoclonal Antibody
