B3GNT1 (N-acetyllactosaminide beta-1,3-N-acetylglucosaminyltransferase, I-beta-1,3-N-acetylglucosaminyltransferase, iGnT, Poly-N-acetyllactosamine Extension Enzyme, UDP-GlcNAc:betaGal beta-1,3-N-acetylglucosaminyltransferase 1, B3GNT6)
Product Sizes
100ug
£748.00
B0010-50-100UG
About this Product
- SKU:
- B0010-50
- Application:
- Western Blot
- translate.label.attr.clone:
- 12K14
- Clonality:
- Monoclonal
- Extra Details:
- B3GNT1 is a 50kD member of the b3GnT family of enzymes. It is synthesized by sensory neurons, endothelial cells and skeletal muscle. In the trans-Golgi, it collaborates with B4GALT1 to generate a linear poly-N-acetyllactosamine sequence that can be incorporated into either N-linked or O-linked glycans. Human B3GNT1 is a 451aa type II transmembrane protein. It contains an 8aa N-terminal cytoplasmic segment, an extended 28aa transmembrane region aa9-36, and a 379aa luminal domain aa37-415. Over aa37-415, human B3GNT1 shares 97% aa identity with mouse B3GNT1. Applications: Suitable for use in Western Blot. Other applications not tested. Recommended Dilution: Western Blot: 2ug/ml Optimal dilutions to be determined by the researcher. Storage and Stability: Lyophilized and reconstituted products are stable for 12 months after receipt at -20°C. Reconstitute with sterile PBS. Aliquot to avoid repeated freezing and thawing. Store at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
- Host:
- Mouse
- Immunogen:
- Recombinant protein corresponding to Asp43-Cys415 from human B3GNT1 expressed in CHO cell line.
- Isotype:
- IgG2b
- Physical State:
- Supplied as a lyophilized powder from PBS, 5% trehalose. Reconstitute with 200ul sterile PBS.
- Purity:
- Purified by Protein A affinity chromatography.
- Shipping Conditions:
- Blue Ice
- Specificity:
- Recognizes human B3GNT1.
- Source:
- human
- Storage Conditions:
- -20[o]C
- Type:
- Antibodies:Monoclonal Antibody
