ASAHL (N-Acylsphingosine Amidohydrolase-like)
Product Sizes
100ug
£825.00
A3597-42A-100UG
About this Product
- SKU:
- A3597-42A
- Application:
- Immunoprecipitation, Western Blot
- translate.label.attr.clone:
- 9H66
- Clonality:
- Monoclonal
- Extra Details:
- ASAHL (N-acylsphingosine-amidohydrolase-like; also acid ceramidase-like protein) is a 30kD member of the acid ceramidase family. It is a secreted lysosomal glycoprotein that cleaves fatty acid amides such as palmitoylethanolamine. Human ASAHL is synthesized as a 359aa preproprecursor that contains a 28aa signal sequence, a 96aa prosegment, and a 30-31kD, 235 aa mature region that begins at Cys125. Multiple isoforms likely exist. One isoform shows a deletion of aa324-359, while another has a two aa substitution for aa 198-359. The human proform (aa29-359) is 78% aa identical to mouse ASAHL proform. Applications: Suitable for use in ELISA, Western Blot, and Immunoprecipitation. Other applications not tested. Recommended Dilution: ELISA: 0.5-1.0ug/ml Western Blot: 1-2ug/ml Immunoprecipitation: 25ug/ml Optimal dilutions to be determined by the researcher. Storage and Stability: Lyophilized powder may be stored at 4°C for short-term only. Reconstitute to nominal volume by adding sterile 40-50% glycerol and store at -20°C. Reconstituted product is stable for 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
- Host:
- Mouse
- Immunogen:
- CHO-derived, recombinant human ASAHL aa29-359; Q02083.
- Isotype:
- IgG1
- Physical State:
- Supplied as a lyophilized powder in 0.2um sterile-filtered solution, PBS, 5% trehalose. Reconstitute with 40-50% glycerol, PBS.
- Purity:
- Purified by Protein G affinity chromatography.
- Shipping Conditions:
- Blue Ice
- Specificity:
- Recognizes human ASAHL.
- Source:
- human
- Storage Conditions:
- -20[o]C
- Type:
- Antibodies:Monoclonal Antibody
