Apolipoprotein B (Apo B)
Product Sizes
100ug
£869.00
A2299-41C-100UG
About this Product
- SKU:
- A2299-41C
- Application:
- Western Blot, Immunohistochemistry
- translate.label.attr.clone:
- 7H145
- Clonality:
- Monoclonal
- Extra Details:
- Human ApoB (apolipoprotein B-100) is a 550kD, secreted, palmitoylated glycoprotein that is part of LDL and VLDL particles. It is made by liver and is 4536aa in length. It binds LDL to the ApoB/E receptor. Multiple forms of ApoB-100 exist; ApoB-25, 26, 29, 37, 39, 48 and 74. The numbers reflect each molecule’s predicted MW as a percentage of ApoB-100 (= 512kD). AA1206-1414 of the precursor were used for immunization. Based on likelihood of secretion, this antibody may recognize B26 (1297aa), B37 (1728aa), B39 (1799aa) and B48 (2152aa). Over the immunization polypeptide range, human ApoB-100 is 80% and 82% aa identical to mouse and canine ApoB-100, respectively. Applications: Suitable for use in Western Blot, Immunohistochemistry and ELISA. Other applications not tested. Recommended Dilutions: Western Blot: 2ug/ml detects ApoB in human plasma Immunohistochemistry (Paraffin): 8-25ug/ml detected A2299-41C in immersion fixed paraffin-embedded sections of human liver Opimal dilutions to be determined by the researcher. Storage and Stability: Lyophilized and reconstituted products are stable for 12 months after receipt at -20°C. Reconstitute with sterile PBS. Aliquot to avoid repeated freezing and thawing. Store at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
- Host:
- Mouse
- Immunogen:
- Recombinant protein corresponding to Met1206-Asp1413 from human Apolipoprotein B (Apo B) expressed in E. coli.
- Isotype:
- IgG2b
- Physical State:
- Supplied as a lyophilized powder from PBS, 5% trehalose. Reconstitute with 200ul sterile PBS.
- Purity:
- Purified by Protein G affinity chromatography from hybridoma culture supernatant.
- Shipping Conditions:
- Blue Ice
- Specificity:
- Recognizes human Apo B.
- Source:
- human
- Storage Conditions:
- -20[o]C
- Type:
- Antibodies:Monoclonal Antibody
