APE (Ref-1, Apurinic Apyrimidinic Endonuclease, APEX, APE-1, HAP1)
Product Sizes
100ug
£672.00
A2298-74-100UG
About this Product
- SKU:
- A2298-74
- Application:
- Western Blot, Immunohistochemistry, Immunofluorescence
- translate.label.attr.clone:
- 3C107 (13B8E5C2)
- Clonality:
- Monoclonal
- Extra Details:
- Mammalian apurinic/apyrimidinic (AP) endonuclease (APE1; APEX; HAP1; Ref-1) is a multifunctional, bipartite enzyme that belongs to the class II AP endonucleases and that plays an important role in numerous, cellular functions, like repairing abasic sites (loss of purine or pyrimidine base) in DNA, regulating the redox state of other proteins that play roles in oxidative signalling, transcription factor regulation (Fos, Jun, NF-kB, Myb, HIF-1a, CREB, Pac), cell cycle control (p53) and apoptosis. APE1 initiates the DNA base excision repair (BER) by cleaving the DNA immediately adjacent to the 5’ of an AP site to produce a hydroxyl group at the 3’ terminus of an unmodified nucleotide upstream of the nick and a 5’-deoxyribose phosphate moiety downstream. The product of APE1 is further processed by DNA polymerase beta to release 5’-deoxyribose phosphate with its intrinsic lyase activity. The nicked DNA is then sealed by DNA ligase I or DNA ligaseIII/XRCC1 to complete this repair process. In addition to the AP endonuclease activity, APE1 also possesses a 3’-5’ DNA exonuclease activity, a 3’-phosphodiesterase activity, a 3’-phosphatase activity, and a RNase H activity. It has been shown that APE1 is capable of excision L-configuration deoxyribonucleoside analogs from the 3’ termini of DNA. Human APE1 gene is located on chromosome 14q 11.2-12. Applications: Suitable for use in Immunocytochemistry/Immunofluorescence, Immunohistochemistry, Immunoprecipitation and Western Blot. Other applications not tested. Recommended Dilutions: Immunohistochemistry (paraffin): 1:100. Western Blot: 1:1000. Detects a band of ~37kD. Immunofluorescence (IC): 1:50-1:200 Optimal dilutions to be determined by the researcher. Positive Control: HeLa Whole Cell Lysate Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
- Host:
- Mouse
- Immunogen:
- Purified human APE/ref protein. Epitope: amino acids 80-100. Cellular Localization: In Immunohistochemistry, punctate nuclear staining is observed in most cell types. Cytoplasmic staining is seen in hepatocytes, large neurons and granule cells.
- Isotype:
- IgG2b
- Physical State:
- Supplied as a liquid in PBS, 0.02% sodium azide.
- Purity:
- Purified by Protein G affinity chromatography.
- Shipping Conditions:
- Blue Ice
- Specificity:
- Recognizes human APE/ref-1 protein. Species Crossreactivity: mouse and rat.
- Source:
- human
- Storage Conditions:
- -20[o]C
- Supplier:
- United States Biological
- Type:
- Antibodies:Monoclonal Antibody
