Anion Exchanger 4 (AE4) (Not for Export EU)
Product Sizes
100ul
£704.00
A2295-14G-100UL
About this Product
- SKU:
- A2295-14G
- Clonality:
- Polyclonal
- Extra Details:
- Anion exchangers (AE) are membrane proteins involved in the regulation of intracellular pH, cell volume regulation as well as in transepithelial acid/base transport. AE proteins are sodium-independent exchangers that mediates one-for-one exchange of extracellular Cl- for intracellular HCO3- ions resulting in intracellular acidification. AE proteins are encoded by a family of at least three related genes (AE1, AE2, and AE3). Numerous alternatively spliced isoforms of each AE gene are expressed in various tissues. AE proteins are exemplified by a large N-terminal cytoplasmic domain (~40-75kD) that provides binding sites for cytoskeleton protein, glycolytic enzymes and hemoglobin. The N-terminal cytoplasmic domains of AE2 are AE3 are more closely related than AE1. In fact, AE1 N-terminus is 300aa shorter than both the AE2 and AE3. The C-terminal TM domain (~55kD) is highly conserved (~70% identity) among various AE, spans the lipid bilayer 12-14 times, and is able to mediate anion exchange by itself. Recently, a new member of AE family, AE4, has been cloned from rabbit and human. Human AE4 (945aa; chromosome 5) is predicted to have 14 TM domains. It is strongly expressed in the kidney. Rabbit and human AE4 share 84% identity. Applications: Suitable for use in ELISA. Western Blot, though not tested, may potentially be used as an application. Other applications not tested. Recommended Dilution: ELISA: 1:10,000-50,000. Coat ELISA plates at 1ug/ml with A2295-14G2-Anion Exchanger 4, Control Peptide Human. Western Blot: 1:1000-1:5000 Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4°C for short-term only. For long-term storage, store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
- Host:
- Rabbit
- Immunogen:
- Synthetic peptide corresponding to 15aa near the cytoplasmic C-terminus of human AE4 (KLH).
- Isotype:
- IgG
- Physical State:
- Supplied as a liquid in PBS, 0.05% sodium azide, before the addition of glycerol to 40%.
- Purity:
- Serum
- Shipping Conditions:
- Blue Ice
- Specificity:
- Recognizes human AE4. Sequence Homology: Human isoforms 1AE, 2, 3, NBC5 (AB032762, 657aa) and NBC (AF313465, 990aa; XM_038736, 959aa) - 100%. Species Sequence Homology: Rabbit and rat AE4 - 100%.
- Source:
- human
- Storage Conditions:
- 4[o]C (-20[o]C Glycerol)
- Supplier:
- United States Biological
- Type:
- Antibodies:Polyclonal Antibody
