Anion Exchanger 1 (AE1, Band3)
Product Sizes
100ul
£927.00
A2295-13O-100UL
About this Product
- SKU:
- A2295-13O
- Application:
- Western Blot
- translate.label.attr.clone:
- 3H3
- Clonality:
- Monoclonal
- Extra Details:
- Anion exchangers (AE) are membranes proteins involved in the regulation of intracellular pH, cell volume regulation as well as in transepithelial acid/base transport. AE proteins are sodium-independent exchangers that mediates one-for-one exchange of extracellular Cl- for intracellular HCO3- ions resulting in intracellular acidification. AE proteins are encoded by a family of at least three related genes (AE1, AE2 and AE3). Numerous alternatively spliced isoforms of each AE gene are expressed in various tissues. AE proteins are exemplified by a large N-terminal cytoplasmic domain (~40-75kD) that provides binding sites for cytoskeleton protein, glycolytic enzymes and hemoglobin. The N-terminal cytoplasmic domains of AE2 are AE3 are more closely related than AE1. In fact, AE1 N-terminus is 300aa shorter than both the AE2 and AE3. The C-terminal TM domain (~55kD) is highly conserved (~70% identity) among various AE, spans the lipid bilayer 12-14 times. It is able to mediate anion exchange by itself. AE1 protein (also know as band 3) is the most abundant protein in red cell membrane. AE1 gene has been cloned and characterized from a variety of species including mouse, rat, human and chicken. Human AE1 gene (chromosome 17q21-qter) encodes for a protein of 911aa (rat 927aa; mouse 929aa). Several isoforms of AE1 are also transcribed. AE1 is primarily expressed in red cells and kidney. AE1 is dimeric in solution. Defects in AE1/band3 gene are the cause of hereditary ovalocystosis (HO) - a disease associated with high degree of membrane rigidity. Applications: Suitable for use in ELISA and Western Blot. Other applications not tested. Recommended Dilutions: ELISA: 1:10,000-50,000 Western Blot: 1:1000-1:5000. AE1 is ~90-100kD protein. It aggregates easily forming dimers and multimers. Aggregation may be avoided if membranes are prepared in N-ethylmaleimide. It may also be proteolytically processed to smaller fragments of 60, 40 and 20kD. Optimal dilutions to be determined by the researcher. Storage and Stability: Lyophilized and reconstituted products are stable for 12 months after receipt at -20°C. Reconstitute with sterile dH2O. Aliquot to avoid repeated freezing and thawing. Store at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
- Host:
- Mouse
- Immunogen:
- Purified human Anion Exchanger 1 (AE1, Band3). Epitope: Located within the 20kD region from the N-terminus domain.
- Isotype:
- IgG2a
- Physical State:
- Supplied as a lyophilized powder, 0.05% sodium azide. Reconstitute with 100ul sterile PBS.
- Purity:
- Ascites
- Shipping Conditions:
- Ambient
- Specificity:
- Recognizes human Anion Exchanger 1 (AE-1, Band3). Does not recognize non-erythroid band-3 from human fibroblasts. Does not recognize band-3 from equine, mouse, bovine, porcine, guinea pig, canine or mouse erythrocytes.
- Source:
- human
- Storage Conditions:
- -20[o]C
- Supplier:
- United States Biological
- Type:
- Antibodies:Monoclonal Antibody
