β-Amyloid 1-40, 42
Product Sizes
100ul
£927.00
A2275-75K-100UL
About this Product
- SKU:
- A2275-75K
- Application:
- Western Blot, Immunohistochemistry
- translate.label.attr.clone:
- 3H2
- Clonality:
- Monoclonal
- Extra Details:
- Alzheimer's Disease (AD) is a neurodegenerative disorder characterized by progressive loss of memory and cognition in the elderly. One of the most important and initial step involves proteolytic cleavage of amyloid precursor protein (APP, chromosome 21) releasing short 40, 42 & 43aa peptides (beta amyloid 1-40, 1-42 and 1-43). Polymerization of b-amyloid (Ab) and subsequent neuronal deposit (amyloid) leads to the degeneration of neurons involved in memory and cognition. Ab deposits have also been found to contain 2 additional proteins termed a-synuclein and b-synuclein. The 140aa a-synucleins is identical with non- Ab component (NACP) of AD. The 134aa human b-synuclein is homologous to 14kD bovine phosphoneuroprotein 14. Mutations in a-synuclein gene causing a replacement of alanine with a threonine may cause the protein to misfold. Synucleins are primarily expressed in the brain. At least 3 forms: two large (140aa SYN-1 & 149aa SYN-2) and a small form (SYN-3, 42aa) are produced by alternative splicing. Applications: Suitable for use in ELISA, Western Blot and Immunohistochemistry. Other applications not tested. Recommended Dilutions: ELISA: 1:10,000-1:100,000 using 50-100ng of control peptide/well. Western Blot: 1:1000-1:5000 (ECL) Immunohistochemistry: 1:500-1000 using formalin or paraformaldehyde-fixed Alzheimers brains. Optimal dilutions to be determined by the researcher. Recommended Control Peptide: Full length b-amyloid (1-40aa and 1-42aa) Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
- Host:
- Mouse
- Immunogen:
- Human beta Amyloid 1-40, conjugated to KLH. Epitope: Localized to the N-terminus of beta amyloid (1-13aa of 1-40/42/43). Species Sequence Homology: mouse, rat and many other species.
- Isotype:
- IgG1
- Physical State:
- Supplied as a liquid, 0.05% sodium azide.
- Purity:
- Ascites.
- Shipping Conditions:
- Blue Ice
- Specificity:
- Recognizes human b-amyloid 1-40/42.
- Source:
- human
- Storage Conditions:
- -20[o]C
- Type:
- Antibodies:Monoclonal Antibody
