Amylin (DAP, Diabetes-Associated Peptide, IAP, Islet Amyloid Polypeptide, Insulinoma Amyloid Peptide, IAPP)
Product Sizes
100ug
£754.00
A2275-53C-100UG
About this Product
- SKU:
- A2275-53C
- Clonality:
- Polyclonal
- Extra Details:
- The calcitonin family of bioactive peptides comprises of calcitonin, amylin, two calcitonin-gene related peptides (CGRP1, and CGRP2) and adrenomedullin (ADM). Calcitonin is 32 aa peptide found in the parafollicular "C" cells of the thyroid in mammals. It is also found in a number of non-mammals. It regulates the mineral (calcium and phosphate) balance. Calcitonin causes hypercalcemia by acting as an inhibitor of osteoclast induced bone resorption. CGRP is a 37-aa peptide produced by tissue specific processing of the calcitonin gene. Calcitonin is the major product in the thyroid, whereas CGRP is the major product in neural tissues. CGRP is a potent cardiovascular agent. It has structural similarity with amylin. CGRP is found in two isoforms (CGRP-I and CGRP-II) that differs only by 3 amino acids. Amylin is a 37-aa peptide produced in the pancreatic beta-cell secretory granules and is co-released with insulin. Amylin also has CGRP-like effects on bone metabolism. Amylin has specific binding sites in the CNS and it may regulate gastric emptying and influence carbohydrate metabolism. Adrenomedullin (ADM) is a 52-aa hypotensive peptide. It has structural similarity with CGRP and amylin. ADM is produced in peripheral tissues, adrenal medulla, lung, and kidney. Applications: Suitable for use in ELISA. Western Blot, though not tested, may potentially be used as an application. Other applications not tested. Recommended Dilutions: ELISA: 0.5-1ug/ml. Control peptide can be used to coat plates at 1ug/ml. Optimal dilutions to be determined by the researcher. Control Peptide: A2275-53D: Amylin, Human Storage and Stability: Lyophilized and reconstituted products are stable for 12 months after receipt at -20°C. Reconstitute with sterile PBS. Aliquot to avoid repeated freezing and thawing. Store at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
- Host:
- Rabbit
- Immunogen:
- Synthetic peptide corresponding to 13aa (aa18-30) from the middle region of human Amylin, conjugated to KLH. . No significant sequence homology with other calcitonin family of peptides. UniProt accession #P10997. Species Sequence Homology: <50% mouse, rat
- Isotype:
- IgG
- Physical State:
- Supplied as a liquid in PBS, pH 7.5, 0.1% BSA.
- Purity:
- Purified by immunoaffinity chromatography.
- Shipping Conditions:
- Blue Ice
- Specificity:
- Recognizes human amylin.
- Source:
- human
- Storage Conditions:
- -20[o]C
- Supplier:
- United States Biological
- Type:
- Antibodies:Polyclonal Antibody
