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Antibodies

AgRP (Agouti Related Protein, ART, AGRT, ASIP2, Agouti-Related Transcript)

Product Sizes
500ug
£1017.00
A1059-62B-500UG
About this Product
SKU:
A1059-62B
Application:
Western Blot
translate.label.attr.clone:
9H47
Clonality:
Monoclonal
Extra Details:
AgRP, also known as the protein product of the Agouti related transcript (ART), functions as an antagonist of the melanocortin (MC-3 and MC-4) receptors, both of which are implicated in obesity. AgRP levels are significantly elevated in the serum of obese mice and people. Applications: Suitable for use in Direct ELISA and Western Blot. Other applications not tested. Recommended Dilutions: Western Blot: 1ug/ml using recombinant human AgRP/ART Optimal dilutions to be determined by the researcher. Storage and Stability: Lyophilized and reconstituted products are stable for 12 months after receipt at -20°C. Reconstitute with sterile PBS. Aliquot to avoid repeated freezing and thawing. Store at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Host:
Mouse
Immunogen:
Recombinant protein corresponding to Ala21-Thr132 from human Agouti-Related Protein, expressed in Sf 21 cells.
Isotype:
IgG2b
Physical State:
Supplied as a lyophilized powder from PBS, 5% trehalose. Reconstitute with 1ml sterile PBS.
Purity:
Purified by Protein A affinity chromatography from hybridoma culture supernatant.
Shipping Conditions:
Blue Ice
Specificity:
Recognizes human AgRP. Shows ~10% crossreactivity with recombinant mouse AgRP in direct ELISA.
Source:
human
Storage Conditions:
-20[o]C
Type:
Antibodies:Monoclonal Antibody