Adipsin (Factor D) (Not for Export EU)
Product Sizes
100ul
£704.00
A0890A-100UL
About this Product
- SKU:
- A0890A
- Clonality:
- Polyclonal
- Extra Details:
- Adipsin is serine protease that is secreted by adipocytes. It is deficient in several animal model of obesity. Adipsin has now been identified as the same protein as complement factor D. Adipsin, also called ADN or complement factor D or C3 convertase activator or properdin factor D (precursors: mouse 259-aa; rat 263 aa, human 253 aa, mature protein 26-253, ~22kD) cleaves factor B when the latter is complexed with factor C3B, activating the C3BB complex, which then becomes C3 convertase of the alternative pathway. Adipocyte is the major protein secreted by the adipocytes. Unlike rodents, adipsin is also expressed in monocytes/macrphages. Most adipsin is secreted in blood (50ug/ml in normal lean mice and 50-100 fold less in fat from db/db or ob/ob or MSG (monosodium glutamate-treated mice). Its expression is induced upon differentiation of preadipocytes. Applications: Suitable for use in ELISA. Western Blot, though not tested, may potentially be used as an application. Other applications not tested. Recommended Dilutions: Western Blot: 1:1000-1:5000. Adipsin is ~22kD. ELISA: 1:10-50,000; 10-100ng of control peptide/well). Optimal dilutions to be determined by the researcher. Recommended Control Peptide: A0890-01: Adipsin Human Control Peptide Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
- Host:
- Rabbit
- Immunogen:
- Synthetic peptide corresponding to 18aa near the N-Terminus of human adipsin, conjugated to KLH. Species Sequence Homology: chimp; 95%, bovine; 80%, porcine; 78%, rat; 0%, mouse; 63%.
- Isotype:
- IgG
- Physical State:
- Supplied as a liquid in PBS, pH 7.2, 0.05% sodium azide.
- Purity:
- Serum
- Shipping Conditions:
- Blue Ice
- Specificity:
- Recognizes human Adipsin.
- Source:
- human
- Storage Conditions:
- -20[o]C
- Supplier:
- United States Biological
- Type:
- Antibodies:Polyclonal Antibody
