Acid Sensitive Ion Channel 3 (ASIC3, DRASIC, Dorsal Root Ganglion ASIC) (Not for Export EU)
Product Sizes
100ul
£704.00
A0576-05-100UL
About this Product
- SKU:
- A0576-05
- Clonality:
- Polyclonal
- Extra Details:
- Tissue acidosis that occurs in ischemia, tissue damage or inflammation is accompanied by pain. Proton-gated cation channels are activated by low pH in nociceptive neurons. H+-gated channels are members of the NaC/DEG superfamily that include: (1) Amiloride-sensitive epithelial Na+ channels (a, b, and g, and d-ENaC subunits); (2) A FMRFamide-gated channel (FaNaC), (3) and mechanosensory channel proteins of nematode degenerins (DEG). NaC/DEG superfamily is characterized by intracellular N and C-termini, two transmembrane domains, and a large extracellular loop. All members of this family are selective for Na+ and blocked by amiloride. The mammalian homolog of degenerins (MDEG or MDEG1; now designated ASIC for Acid Sensing Ion Channels). Three are at least three distinct proteins in ASIC family: ASIC1 (identical with human BNAC2 or BNC2), expressed in brain and dorsal root ganglions (DRG) cells, is activated by pH <7.0. Applications: Suitable for use in ELISA. Western Blot, though not tested, may potentially be used as an application. Other applications not tested. Recommended Dilution: Western Blot: 1:1000-1:5000 using ECL. ELISA: 1:10,000-1:50,000. Coat ELISA plates at 1ug/ml with Control Peptide. Optimal dilutions to be determined by the researcher. Recommended Control Peptide: A0576-06: Acid Sensitive Ion Channel 3, Rat, CT, 21aa (ASIC3, DRASIC, Dorsal Root Ganglion ASIC) Control Peptide. Storage and Stability: May be stored at 4°C for short-term only. For long-term storage, store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
- Host:
- Rabbit
- Immunogen:
- Synthetic peptide corresponding to 21aa sequence near the cytoplasmic C-terminus of rat DRASIC. (KLH coupled).
- Isotype:
- IgG
- Physical State:
- Supplied as a liquid in PBS, 0.05% sodium azide, 40% glycerol.
- Purity:
- Serum
- Shipping Conditions:
- Blue Ice
- Specificity:
- Recognizes rat Acid Sensitive Ion Channel 3. No significant sequence homology with other ASICs. Species sequence homology: human: 95%; human testis specific DRASIC homolog hTNaCl: 90%.
- Source:
- rat
- Storage Conditions:
- 4[o]C (-20[o]C Glycerol)
- Supplier:
- United States Biological
- Type:
- Antibodies:Polyclonal Antibody
