AcCoA Carboxylase 1 (ACC-1, Acetyl Coenzyme A Carboxylase 1) (Not for Export EU)
Product Sizes
100ul
£704.00
A0225-01B-100UL
About this Product
- SKU:
- A0225-01B
- Clonality:
- Polyclonal
- Extra Details:
- In cells, excess of metabolic fuel is converted into fatty acids in cytosol and oxidized later in mitochondria to generate ATP and acetyl-CoA. In fatty acid synthesis, catalytic formation of malonyl-CoA (precursor for long-chain fatty acyl-CoA, LCFA-CoA) from acetyl-CoA by Acetyl-CoA carboxylase (ACC-1) is the rate limiting step. The translocation of LCFA-CoA from cytosol to mitochondria is catalyzed by two carnitine palmitoyl transferases (CPT-1 & CPT-2) and regulated by ACC-2, the rate limiting step of mitochondrial fatty acid b-oxidation. Activities of ACC-1 and 2 are regulated by their phosphorylation by 5'-AMP-activated protein kinase (AMPK). Diabetes deranges AMPK master-switch and represses the ACC-1 gene expression and stimulates excessive fatty acid oxidation which in turn interferes with glucose metabolism. Applications: Suitable for use in ELISA. Western Blot, though not tested, may potentially be used as an application. Other applications not tested. Recommended Dilution: Western Blot: 1:1000-1:5000 ELISA: 1:10,000-1:100,000 using 50-100ng of control peptide/well. Optimal dilutions to be determined by the researcher. Recommended Control Peptide: A0225-01: AcCoA Carboxylase 1, Human, Control Peptide (ACC-1) Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
- Host:
- Rabbit
- Immunogen:
- Synthetic peptide 15aa sequence mapping near the N-terminus of Human ACC-1 (KLH). Species Sequence Homology: mouse, rat, porcine, bovine, sheep: 100%; chicken: 86%.
- Isotype:
- IgG
- Physical State:
- Supplied as a liquid in 0.05% sodium azide.
- Purity:
- Serum
- Shipping Conditions:
- Blue Ice
- Specificity:
- Recognizes human Acetyl-CoA Carboxylase-1 (ACC-1). No significant sequence homology is seen with ACC-2 or any other protein.
- Source:
- human
- Storage Conditions:
- -20[o]C
- Type:
- Antibodies:Polyclonal Antibody
