EGF Receptor
Product Sizes
100ug
£793.00
590163-100UG
About this Product
- SKU:
- 590163
- Application:
- Immunoprecipitation, Flow Cytometry, Western Blot
- translate.label.attr.clone:
- 6F1
- Clonality:
- Monoclonal
- Extra Details:
- The epidermal growth factor receptor (EGFR) is a 170kD membrane bound receptor consisting of an extracellular ligand-binding domain, a single hydrophobic transmembrane region and a tyrosine kinase domain-containing cytoplasmic region. Ligand binding induces receptor homo- or heterodimerization, triggering autophosphorylation of cytoplasmic tyrosine residues that provide docking sites for Src homology 2 (SH2) domain-containing signaling molecules. Src-mediated phosphorylation of EGFR at Tyr845 may contribute to EGFR hyperactivity in cancer, while autophosphorylation of EGFR at Tyr1068 and/or Tyr1173 in response to ligand binding is known to recruit cofactors (e.g. SHP1, RasGAP, SOS1, Grb2) that activate pathways associated with cell growth, apoptosis, adhesion, receptor endocytosis, and protein degradation. Applications: Suitable for use in Flow Cytometry, Western Blot, Immunoprecipitation and Immunocytochemistry. Other applications not tested. Recommended Dilution: Flow Cytometry: 1:100 Western Blot: 1:1000-1:2000 Immunocytochemistry: 1:100 Immunoprecipitation: 2-5ul/250ul of cell extract from 2.5 x 10e6 cells Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
- Host:
- Mouse
- Immunogen:
- Recombinant protein corresponding to human EGF receptor.
- Isotype:
- IgG2b
- Physical State:
- Supplied as a liquid in PBS, pH 7.2, 50% glycerol.
- Purity:
- Purified by ammonium sulfate precipitation from ascites.
- Shipping Conditions:
- Blue Ice
- Specificity:
- Recognizes human EGF receptor.
- Source:
- human
- Storage Conditions:
- -20[o]C
- Supplier:
- United States Biological
- Type:
- Antibodies:Monoclonal Antibody
