KLRG1 (Killer Cell Lectin-like Receptor Subfamily G Member 1, CLEC15A, MAFA, 2F1, MAFA-L, MAFA-2F1)
Product Sizes
100ug
£1017.00
589919-100UG
About this Product
- SKU:
- 589919
- Application:
- Flow Cytometry, Immunohistochemistry
- translate.label.attr.clone:
- 2388C
- Clonality:
- Monoclonal
- Extra Details:
- KLRG1 (killer cell lectin-like receptor G1), also called MAFA (mast cell function associated), is a 30-38 kDa type II transmembrane inhibitory glycoprotein of the C‑type lectin family, designated CLEC15A. KLRG1 cDNA encodes 195 amino acids (aa) including an intracellular ITIM motif and a 136 aa extracellular domain (ECD) with a single C‑type lectin domain. The human KLRG1 ECD shares 57% and 54% aa identity with mouse and rat KLRG1, respectively. A 189 aa isoform diverges at aa 186. KLRG1 binds E-, N- and R-cadherins and functions as an MHC‑independent means of identifying non-self pathogens and epithelial tumor cells with low E‑cadherin expression. It is expressed as a monomer or disulfide‑linked homodimer on NK and T cell subsets such as tumor-infiltrating lymphocytes. Applications: Suitable for use in Flow Cytometry and Immunohistochemistry. Other applications not tested. Recommended Dilutions: Immunohistochemistry (Paraffin): 3-25ug/ml using immersion fixed paraffin-embedded sections of human ronsil Flow Cytometry: 0.25ug/10e6 cells using human peripheral blood Optimal dilutions to be determined by the researcher. Storage and Stability: Lyophilized and reconstituted products are stable for 12 months after receipt at -20°C. Reconstitute with sterile PBS. Aliquot to avoid repeated freezing and thawing. Store at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
- Host:
- Rabbit
- Immunogen:
- Recombinant protein corresponding to Leu60-Phe195 from human KLRG1, expressed in CHO cells.
- Isotype:
- IgG
- Physical State:
- Supplied as a lyophilized powder from PBS, pH 7.4, 5% trehalose. Reconstitute with 200ul sterile PBS.
- Purity:
- Purified by Protein A affinity chromatography from cell culture supernatant.
- Shipping Conditions:
- Blue Ice
- Specificity:
- Recognizes human KLRG1.
- Source:
- human
- Storage Conditions:
- -20[o]C
- Supplier:
- United States Biological
- Type:
- Antibodies:Monoclonal Antibody
