PTEN Induced Putative Kinase 1 (PINK1) BioAssay™ ELISA Kit (Mouse)
Product Sizes
96Tests
£940.00
589684-96TESTS
About this Product
- SKU:
- 589684
- Extra Details:
- The PTEN Induced Putative Kinase 1 (PINK1) BioAssay™ ELISA Kit is a sandwich enzyme immunoassay for in vitro quantitative measurement of PINK1 in mouse tissue homogenates, cell lysates and other biological fluids. Detection Range: 0.156-10ng/ml Sensitivity: 0.061ng/ml Intra-Assay: CV<10% Inter-Assay: CV<12% Kit Components: *589684A: Microtiter Plate, 1x96 wells, Pre-coated, ready to use. *589684B: Standard, 2x1vial 589684C: Standard Diluent, 1x20ml *589684D: Detection Reagent A (green), 1x120ul *589684E: Detection Reagent B (red), 1x120ul 589684F: Assay Diluent A, 1x12ml 589684G: Assay Diluent B, 1x12ml 589684H: TMB Substrate, 1x9ml *589684J: Positive Control, xxng/ml, 1x1vial (Lyophilized) 589684K: Stop Solution, 1x6ml 589684L: Wash Buffer, 30X, 1x20ml Precaution: The Stop Solution (589684K) included for use in this kit is an acid solution. Wear eye, hand, face, and clothing protection when using this material. Storage and Stability: Store *589684A, *589684B, *589684D, *589684E and *589684J at -20°C. After reconstitution of *5896848, store at 4°C and use within 5 days. Store all the other components at 4°C. Unused kit is stable for 6 months after receipt. Once kit components are opened, it is highly recommended to use remaining reagents within 1 month provided this is within the expiration date of the kit. For maximum recovery of product, centrifuge the original vials after thawing and prior to removing the cap. Assay Procedure Summary: 1. Prepare all reagents, samples and standards. 2. Add 100ul standard or sample to each well. Incubate 1 hour at 37°C. 3. Aspirate and add 100ul prepared Detection Reagent A. Incubate 1 hour at 37°C. 4. Aspirate and wash 3 times. 5. Add 100ul prepared Detection Reagent B. Incubate 30 minutes at 37°C. 6. Aspirate and wash 5 times. 7. Add 90ul Substrate Solution. Incubate 10-20 minutes at 37°C. 8. Add 50ul Stop Solution. Read at 450nm immediately.
- Shipping Conditions:
- Blue Ice
- Storage Conditions:
- 4[o]C/-20[o]C
- Type:
- Assay Kits:Assays
