IgE
Product Sizes
100ug
£550.00
553811-100UG
About this Product
- SKU:
- 553811
- Application:
- Western Blot, Immunohistochemistry
- translate.label.attr.clone:
- 7H2
- Clonality:
- Monoclonal
- Extra Details:
- IgE is an immunoglobulin primarily produced from plasma cells and, in normal serum, present at very low concentrations. Western blot analysis against affinity purified equine IgE using 553811 demonstrates a single major band of approximately 200kD under non-reducing conditions that corresponds with the expected molecular weight of the complete equine IgE molecule (Wilson, D.A. et al. 2006). IgE is important in both type 1 hypersensitivity and immunity to parasite infections, in particular parasitic worms where equine IgE levels are significantly elevated following worm infection. Monoclonal antibodies to equine IgE are of particular relevance to research into insect bite sensitivity, one of the most widely studied allergic diseases in equid species. Applications: Suitable for use in ELISA, Western Blot and Immunohistochemistry. Other applications not tested. Recommended Dilution: ELISA: 1:40-1:400 in an indirect ELISA or as a capture antibody with 553813 as the detection reagent. Western Blot: detects a band of ~200kD in non-reducing conditions. Not suitable for use in western blotting under reducing conditions. Optimal dilutions to be determined by the researcher. Hybridoma: Spleen cells from immunized BALB/c mice were fused with cells of the mouse P3X myeloma cell line. Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
- Host:
- Mouse
- Immunogen:
- Equine IgE
- Isotype:
- IgG1
- Physical State:
- Supplied as a liquid in PBS, 0.09% sodium azide.
- Purity:
- Purified by Protein A affinity chromatography from tissue culture supernatant.
- Shipping Conditions:
- Blue Ice
- Specificity:
- Recognizes native equine IgE. Does not cross react with equine IgM, IgA or IgG.
- Source:
- equine
- Storage Conditions:
- -20[o]C
- Supplier:
- United States Biological
- Type:
- Antibodies:Monoclonal Antibody
