Mitochondrial Open Reading Frame Of The 12S rRNA-c (MOTS-c) BioAssay™ ELISA Kit (Human)
Product Sizes
96Tests
£960.00
517443-96TESTS
About this Product
- SKU:
- 517443
- Extra Details:
- The kit is a competitive inhibition enzyme immunoassay technique for the in vitro quantitative measurement of MOTS-c in human serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids. Sensitivity: 0.94ng/ml Range: 2.47-200ng/ml Specificity: This assay has high sensitivity and excellent specificity for detection of Mitochondrial Open Reading Frame Of The 12S rRNA-c (MOTS-c). No significant cross-reactivity or interference between Mitochondrial Open Reading Frame Of The 12S rRNA-c (MOTS-c) and analogues was observed. Precision: Intra-Assay: CV<10% Inter-Assay: CV<12% Test Principle: This assay employs the competitive inhibition enzyme immunoassay technique. A monoclonal antibody specific to MOTS-c has been pre-coated onto a microplate. A competitive inhibition reaction is launched between biotin labeled MOTS-c and unlabeled MOTS-c (Standards or samples) with the pre-coated antibody specific to MOTS-c. After incubation the unbound conjugate is washed off. Next, avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. The amount of bound HRP conjugate is reverse proportional to the concentration of MOTS-c in the sample. After addition of the substrate solution, the intensity of color developed is reverse proportional to the concentration of MOTS-c in the sample. Kit Components: 96-well strip plate, 1x Plate Standard, 2x vial Detection Reagent A, 1 Detection Reagent B, 1×120ul Reagent Diluent, 1×300ul TMB Substrate, 1×9ml Wash Buffer (30X), 1×20ml Standard Diluent, 1×20ml Assay Diluent A, 1×12ml Assay Diluent B, 1×12ml Stop Solution, 1x6ml Storage and Stability: Store all components at 4°C. Stable for 6 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
- Shipping Conditions:
- Blue Ice
- Storage Conditions:
- 4[o]C/-20[o]C
- Type:
- Assay Kits:Assays
