Antidiuretic Hormone (ADH) High Sensitivity BioAssay™ ELISA Kit (Human)
Product Sizes
96Tests
£960.00
516997-96TESTS
About this Product
- SKU:
- 516997
- Extra Details:
- The kit is a competitive inhibition enzyme immunoassay technique for the in vitro quantitative measurement of ADH in in human tissue homogenates, cell lysates and other biological fluids. Sensitivity: 2.92pg/ml Range: 6.17-500pg/ml Specificity: This assay has high sensitivity and excellent specificity for detection of High Sensitive Antidiuretic Hormone (ADH). No significant cross-reactivity or interference between High Sensitive Antidiuretic Hormone (ADH) and analogues was observed. Precision: Intra-Assay: CV<10% Inter-Assay: CV<12% Test Principle: This assay employs the competitive inhibition enzyme immunoassay technique. A monoclonal antibody specific to ADH has been pre-coated onto a microplate. A competitive inhibition reaction is launched between biotin labeled ADH and unlabeled ADH (Standards or samples) with the pre-coated antibody specific to ADH. After incubation the unbound conjugate is washed off. Next, avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. The amount of bound HRP conjugate is reverse proportional to the concentration of ADH in the sample. After addition of the substrate solution, the intensity of color developed is reverse proportional to the concentration of ADH in the sample. Kit Components: 96-well strip plate, 1x Plate Standard, 2x vial Detection Reagent A, 1 Detection Reagent B, 1×120ul Reagent Diluent, 1×300ul TMB Substrate, 1×9ml Wash Buffer (30X), 1×20ml Standard Diluent, 1×20ml Assay Diluent A, 1×12ml Assay Diluent B, 1×12ml Stop Solution, 1x6ml Storage and Stability: Store all components at 4°C. Stable for 6 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
- Shipping Conditions:
- Blue Ice
- Storage Conditions:
- 4[o]C/-20[o]C
- Type:
- Assay Kits:Assays
