CD40 Ligand, Recombinant, Human, aa113-261 (CD40LG)
Product Sizes
10ug
£381.00
516800-10UG
About this Product
- SKU:
- 516800
- Extra Details:
- CD40LG protein is expressed on all B lymphocytes during various stages of development, activated T cells and monocytes, follicular dendritic cells, thymic epithelial cells, and various carcinoma cell lines. It is expressed on most mature B cell malignancies and on some early B cell acute lymphocytic leukemias. Human CD40LG has been found to be the receptor for a protein involved in an X-linked immunodeficiency syndrome. Based on its homology with other members of the TNF ligand protein superfamily, CD40LG is also referred to also as TNFSF5. The soluble form of CD40LG is generated by the intracellular proteolytic processing of the full length CD40LG. Recombinant Human CD40LG is a 17kD protein that represents the soluble segment of full length CD40LG and encompasses the receptor binding TNF-like domain. Partial recombinant protein corresponding to aa113-261 from human CD40 Ligand, expressed in E. coli. Molecular Weight: ~16.2kD Biological Activity: Measured by its binding ability in a functional ELISA the ED50 of human CD40L is 6.05ng/ml. Immobilized Human CD40-Fc at 5ug/ml can bind human CD40L. Amino Acid Sequence: MQKGDQNPQIAAHVISEASSKTTSVLQWAEKGYYTMSNNLVTLENGKQLTVKRQGLYYIYAQVTFCSNREASSQAPFIASLCLKSPGRFERILLRAANTHSSAKPCGQQSIHLGGVFELQPGASVFVNVTDPSQVSHGTGFTSFGLLKL Storage and Stability: Lyophilized and reconstituted products are stable for 6 months after receipt at -20°C. Reconstitute with sterile ddH2O. Aliquot to avoid repeated freezing and thawing. Store at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
- Molecular Weight:
- 16.2
- Physical State:
- Supplied as a lyophilized powder in 20mM PBS, pH 7.0, 0.1mM EDTA. Reconstitute with sterile ddH2O to 0.1-1mg/ml.
- Purity:
- ≥95% (SDS-PAGE). Endotoxin: ≤1EU/ug (LAL).
- Shipping Conditions:
- Blue Ice
- Storage Conditions:
- -20[o]C
- Supplier:
- United States Biological
- Type:
- Proteins, Peptides, Small Molecules & Other Biomolecules:Recombinant Proteins
