Thymidine Kinase 1, Soluble (TK1)
Product Sizes
200ul
£544.00
502734-200UL
About this Product
- SKU:
- 502734
- Application:
- Western Blot, Immunofluorescence
- translate.label.attr.clone:
- C1
- Clonality:
- Monoclonal
- Extra Details:
- Thymidine kinase (EC 2.7.1.21) catalyzes the phosphorylation of thymidine to deoxythymidine monophosphate. Genetic variation in soluble thymidine kinase has not been identified in man. Weiss and Green (1967) found that fusion of mouse cells lacking this enzyme with normal human cells could be achieved, that progressive loss of human chromosomes from the hybrid occurred with passage of time, and that at a stage when only one human chromosome remained the cell still had the capacity to synthesize thymidine kinase. Two forms of Thymidine kinase 1, soluble have been identified in animal cells, one in cytosol and one in mitochondria. Activity of the cytosolic enzyme is high in proliferating cells and peaks during the S-phase of the cell cycle; it is very low in resting cells. Applications: Suitable for use in Western Blot, Immunocytochemistry, Immunofluorescence. Other applications not tested. Recommended Dilutions: Western Blot: 0.01-6ug/ml Immunocytochemistry/Immunofluorescence: 5-20ug/ml Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
- Host:
- Mouse
- Immunogen:
- Recombinant protein corresponding to aa2-234 from human TK1, expressed in E. coli.
- Isotype:
- IgG2b,k
- Physical State:
- Supplied as a liquid in PBS, pH 7.4, 0.05% Proclin-300, 50% glycerol.
- Purity:
- Purified by Protein A/G affinity chromatography.
- Shipping Conditions:
- Blue Ice
- Specificity:
- Recognizes human TK1. Species Crossreactivity: porcine
- Source:
- human
- Storage Conditions:
- -20[o]C
- Supplier:
- United States Biological
- Type:
- Antibodies:Monoclonal Antibody
