PCOLCE, Recombinant, Human, aa26-449, His-Tag (Procollagen C-Endopeptidase Enhancer 1, PCOLCE PCPE, PCPE-1, PCPE1)
Product Sizes
50ug
£607.00
396914-50UG
About this Product
- SKU:
- 396914
- Extra Details:
- PCOLCE, also known as procollagen C-endopeptidase enhancer 1, is a secreted extracellular matrix glycoprotein. This protein is expressed primarily by interstitial connective tissues such as tendons, calvaria, and skin. Also, fibrillar collagen types I-III are synthesized as precursor molecules known as procollagens. These precursors contain amino- and C-terminal peptide extensions known as N- and C-propeptides, respectively, which are cleaved, upon secretion of procollagen from the cell, to yield the mature triple helical, highly structured fibrils. Also, this protein binds and drives the enzymatic cleavage of type 1 procollagen and heightens C-proteinase activity. Recombinant human PCOLCE, fused to His-Tag at C-terminal, expressed in insect cell and purified by using conventional chromatography techniques. Source: Recombinant protein corresponding to aa26-449 of human PCOLCE, fused to His-Tag at C-terminal, expressed in insect cell. Molecular Weight: ~46.6kD (433aa), ~40-57kD (SDS-PAGE under reducing conditions.) Endotoxin: <1EU/1ug (determined by LAL method) Amino Acid Sequence: ADPQTPNYTR PVFLCGGDVK GESGYVASEG FPNLYPPNKE CIWTITVPEG QTVSLSFRVF DLELHPACRY DALEVFAGSG TSGQRLGRFC GTFRPAPLVA PGNQVTLRMT TDEGTGGRGF LLWYSGRATS GTEHQFCGGR LEKAQGTLTT PNWPESDYPP GISCSWHIIA PPDQVIALTF EKFDLEPDTY CRYDSVSVFN GAVSDDSRRL GKFCGDAVPG SISSEGNELL VQFVSDLSVT ADGFSASYKT LPRGTAKEGQ GPGPKRGTEP KVKLPPKSQP PEKTEESPSA PDAPTCPKQC RRTGTLQSNF CASSLVVTAT VKSMVREPGE GLAVTVSLIG AYKTGGLDLP SPPTGASLKF YVPCKQCPPM KKGVSYLLMG QVEENRGPVL PPESFVVLHR PNQDQILTNL SKRKCPSQPV RAAASQDHHH HHH Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing.. Store at -20°C. Aliquots are stable for 6 months after receipt at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
- Molecular Weight:
- 46.6
- Physical State:
- Supplied as a liquid in 20mM Tris-HCl , pH 8.0, 0.1M sodium chloride, 10% glycerol.
- Purity:
- ≥95% (SDS-PAGE)
- Shipping Conditions:
- Blue Ice
- Storage Conditions:
- 4[o]C/-20[o]C
- Type:
- Assay Kits:Collagen
