Serum Amyloid A1/A2, Mouse
Product Sizes
100ug
£978.00
378689-100UG
About this Product
- SKU:
- 378689
- Application:
- Western Blot, Immunohistochemistry
- Clonality:
- Polyclonal
- Extra Details:
- Human serum amyloid A protein-1 (SAA-1) is a multifunctional apolipoprotein produced by hepatocytes in response to proinflammatory cytokines. It is secreted as a 12kD, 104 amino acid (aa), nonglycosylated polypeptide that displaces apoA1 in the HDL3 complex (SwissProt # P02735). The SAA-1 gene is one of three SAA genes in human, and it shows multiple alleles that are race dependent. The SAA-1 gene product differs from the SAA-2 gene product by only seven amino acids. Circulating SAA-1 shows multiple proteolytically-generated isoforms, with anywhere from one-to-three amino acids being cleaved from either the N- or C-terminus. Mature human SAA-1 is 72%, 82% and 72% aa identical to mature mouse, rabbit and hamster SAA-1, respectively. Applications: Suitable for use in Immunohistochemistry, ELISA and Western Blot. Other applications not tested. Recommended Dilutions: Immunohistochemistry (frozen): 5-15ug/ml using mouse liver Western Blot: 1ug/ml using mouse serum and mouse plasma Optimal dilutions to be determined by the researcher. Storage and Stability: Lyophilized and reconstituted products are stable for 12 months after receipt at -20°C. Reconstitute with sterile PBS. Aliquot to avoid repeated freezing and thawing. Store at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
- Host:
- Goat
- Immunogen:
- Recombinant protein corresponding to Gly20-Tyr122 from mouse Serum Amyloid A1, expressed in E. coli.
- Isotype:
- IgG
- Physical State:
- Supplied as a lyophilized powder from PBS, pH 7.4, 5% trehalose. Reconstitute with 500ul sterile PBS.
- Purity:
- Purified by immunoaffinity chromatography.
- Shipping Conditions:
- Blue Ice
- Specificity:
- Recognizes mouse Serum Amyloid A1/SAA1 and mouse Serum Amyloid A2/SAA2. <3% cross-reactivity with recombinant human (rh) SAA1, rhSAA2, recombinant mouse (rm) SAA3, and rmSAA4.
- Source:
- mouse
- Storage Conditions:
- -20[o]C
- Type:
- Antibodies:Sera
