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Antibodies

Keratin K18 (Cytokeratin 18, CYK18, K18)

Product Sizes
5ml
£723.00
367628-5ML
About this Product
SKU:
367628
Application:
Western Blot, Immunohistochemistry, Immunofluorescence
translate.label.attr.clone:
Ks18.5
Clonality:
Monoclonal
Extra Details:
Keratin 18 is a type I cytokeratin. It is, together with its filament partner keratin 8, perhaps the most commonly found products of the intermediate filament gene family. They are expressed in single layer epithelial tissues of the body. Mutations in this gene have been linked to cryptogenic cirrhosis. Two transcript variants encoding the same protein have been found for this gene. Keratin 18 is often used together with keratin 8 and keratin 19 to differentiate cells of epithelial origin from hematopoietic cells in tests that enumerate circulating tumor cells in blood. Represents an excellent marker to discriminate simple epithelia from those of different origin. Applications: Suitable for use in Immunocytochemistry/Immunofluorescence, ELISA, Immunohistochemistry and Western Blot. Other applications not tested. Recommended Dilution: Immunohistochemistry: frozen Optimal dilutions to be determined by the researcher. Reactivity on Cultured Cell Lines: MCF-7, HeLa. A-431, PLC, RT 112. Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Host:
Mouse
Immunogen:
Cytoskeletal preparation from human tumor cell line MCF-7
Isotype:
IgG1
Physical State:
Supplied as a liquid in 0.09% sodium azide.
Purity:
Supernatant
Shipping Conditions:
Blue Ice
Specificity:
Recognizes human Keratin K18. Species Crossreactivity: bovine. Does not react with mouse.
Source:
human
Storage Conditions:
-20[o]C
Supplier:
United States Biological
Type:
Antibodies:Monoclonal Antibody