OGG1 (Oxoguanine Glycosylase 1, HMMH, HOGG1, MUTM, OGH1, 8-Oxoguanine DNA Glycosylase, DNA-(apurinic or apyrimidinic site) lyase, N-glycosylase/DNA lyase)
Product Sizes
200ul
£659.00
364483-200UL
About this Product
- SKU:
- 364483
- Application:
- Western Blot, Immunofluorescence
- Clonality:
- Polyclonal
- Extra Details:
- OGG1 is the primary enzyme responsible for the excision of 7,8-dihydro-8-oxoguanine (8-oxoG), a mutagenic base byproduct which occurs as a result of exposure to reactive oxygen species (ROS). The action of this enzyme includes lyase activity for chain cleavage. Alternative splicing of the C-terminal region of this gene classifies splice variants into two major groups, type 1 and type 2, depending on the last exon of the sequence. Type 1 alternative splice variants end with exon 7 and type 2 end with exon 8. All variants share the N-terminal region in common. Many alternative splice variants for this gene have been described, but the full-length nature for every variant has not been determined. The N-terminus of this gene contains a mitochondrial targeting signal, essential for mitochondrial localization. Applications: Suitable for use in Immunocytochemistry/Immunofluorescence and Western Blot. Other applications not tested. Recommended Dilution: Immunocytochemistry/Immunofluorescence: 5-20ug/ml Western Blot: 0.01-2ug/ml Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
- Host:
- Rabbit
- Immunogen:
- Recombinant protein corresponding to aa31-345 of human OGG1, expressed in E. coli.
- Isotype:
- IgG
- Physical State:
- Supplied as a liquid in PBS, pH 7.4, 0.05% Proclin-300, 50% glycerol.
- Purity:
- Purified by Protein A and peptide affinity chromatography.
- Shipping Conditions:
- Blue Ice
- Specificity:
- Recognizes human OGG1. Species Crossreactivity: mouse and porcine
- Source:
- human
- Storage Conditions:
- -20[o]C
- Type:
- Antibodies:Polyclonal Antibody
