NUP85 (Nucleoporin 85kD, Nup75, FROUNT, Nuclear pore complex protein Nup85, Pericentrin-1)
Product Sizes
200ul
£559.00
364461-200UL
About this Product
- SKU:
- 364461
- Application:
- Western Blot, Immunohistochemistry, Immunofluorescence
- Clonality:
- Polyclonal
- Extra Details:
- Bidirectional transport of macromolecules between the cytoplasm and nucleus occurs through nuclear pore complexes (NPCs) embedded in the nuclear envelope. NPCs are composed of subcomplexes, and NUP85 is part of one such subcomplex, Nup107-160. By proteomic analysis and mass spectrometry, Cronshaw et al. (2002) identified 94 proteins associated with NPCs purified from rat liver nuclei. Database analysis identified FLJ12549, which Cronshaw et al. (2002) called NUP75, as the human homolog of a rat protein found in relative abundance, with 16 copies per NPC. The deduced human protein has a calculated molecular mass of 75kD and is homologous to the yeast Nup85 protein. Applications: Suitable for use in Western Blot, Immunohistochemistry, Immunofluorescence and Immunocytochemistry. Other applications not tested. Recommended Dilutions: Western Blot: 0.01-2ug/ml Immunohistochemistry (Paraffin): 5-20ug/ml Immunocytochemistry/Immunofluorescence: 5-20ug/ml Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
- Host:
- Rabbit
- Immunogen:
- Recombinant protein corresponding to aa418-653 of mouse NUP85, expressed in E. coli.
- Isotype:
- IgG
- Physical State:
- Supplied as a liquid in PBS, pH 7.4, 0.02% sodium azide, 50% glycerol.
- Purity:
- Purified by Protein A and peptide affinity chromatography.
- Shipping Conditions:
- Blue Ice
- Specificity:
- Recognizes mouse NUP85. Species Crossreactivity: human
- Source:
- mouse
- Storage Conditions:
- -20[o]C
- Supplier:
- United States Biological
- Type:
- Antibodies:Polyclonal Antibody
