NOS3 (Nitric Oxide Synthase 3, Endothelial, ECNOS, NOS III, E-NOS, C-NOS, ENOS, CNOS, Constitutive NOS)
Product Sizes
200ul
£473.00
364390-200UL
About this Product
- SKU:
- 364390
- Application:
- Immunoprecipitation, Immunohistochemistry, Western Blot
- Clonality:
- Polyclonal
- Extra Details:
- Nitric oxide (NO) is produced by a group of enzymes called nitric oxide synthases (NOS). These enzymes catalyze the production of NO and L-citrulline from L-arginine, O2, and NADPH-derived electrons. The cell types that express eNOS include vascular endothelial cells, cardiomyocytes and others. In blood vessels, NO produced by the eNOS in endothelial cells functions as a vasodilator thereby regulating blood flow and pressure. Mutant eNOS knockout mice have blood pressure that is 30% higher than wild-type littermates. Within cardiomyocytes, eNOS affects Ca2 currents and contractility. Expression of eNOS is usually reported to be constitutive, though modest degrees of regulation occur in response to factors such as shear stress, exercise, chronic hypoxia, and heart failure. Applications: Suitable for use in Western Blot, Immunohistochemistry, Immunocytochemistry and Immunoprecipitation. Other applications not tested. Recommended Dilution: Western blotting: 0.5-2ug/ml. Immunohistochemistry: 5-20ug/ml Immunocytochemistry: 5-20ug/ml Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
- Host:
- Rabbit
- Immunogen:
- Recombinant protein corresponding to aa522-705 of canine NOS3, expressed in E. coli.
- Isotype:
- IgG
- Physical State:
- Supplied as a liquid in PBS, pH 7.4, 0.02% sodium azide, 50% glycerol.
- Purity:
- Purified by Protein A and peptide affinity chromatography.
- Shipping Conditions:
- Blue Ice
- Specificity:
- Recognizes canine NOS3. Species Crossreactivity: human, mouse and rat
- Source:
- canine
- Storage Conditions:
- -20[o]C
- Supplier:
- United States Biological
- Type:
- Antibodies:Polyclonal Antibody
