MMP3 (Matrix Metalloproteinase 3, SL1, STMY, STMY1, STR1, Progelatinase, Stromelysin 1, Transin-1)
Product Sizes
200ul
£466.00
364184-200UL
About this Product
- SKU:
- 364184
- Application:
- Western Blot, Immunohistochemistry
- Clonality:
- Polyclonal
- Extra Details:
- MMP-3 is secreted from the cells as a proenzyme. The proenzyme has been shown to stimulate plasminogen activation. The N-terminal pro-domain contains the cysteine switch motif conserved in MMPs that maintains MMP-3 in the latent state. Activation of the proenzyme results in the removal of the pro-domain. MMP-3 activation can be achieved in vitro by proteases such as itself, chyrotrypsin, neutrophil elastase and plasma kallikrein, and by mercury compounds. The resulting active enzyme consists of a catalytic domain with a zinc-binding motif conserved in metzincins. A short hinge peptide links the catalytic domain to the C-terminal hemopexin-like domain. The active MMP-3 is capable of cleaving types III, IV, IX and X collagen, aggrecan, fibronectin, laminin, IGFBP-3, serpins, and IL-1. Applications: Suitable for use in Western Blot and Immunohistochemistry. Other applications not tested. Recommended Dilution: Western Blot: 0.01-2ug/ml Immunohistochemistry: 5-30ug/ml Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap
- Host:
- Rabbit
- Immunogen:
- Recombinant protein corresponding to aa247-370 from human MMP3, expressed in E. coli.
- Isotype:
- IgG
- Physical State:
- Supplied as a liquid in PBS, pH 7.4, 0.05% Proclin-300, 50% glycerol.
- Purity:
- Purified by Protein A and peptide affinity chromatography.
- Shipping Conditions:
- Blue Ice
- Specificity:
- Recognizes human MMP3. Species Crossreactivity: mouse, rat and porcine
- Source:
- human
- Storage Conditions:
- -20[o]C
- Supplier:
- United States Biological
- Type:
- Antibodies:Polyclonal Antibody
