LY75 (Lymphocyte Antigen 75, CD205, LY75, CLEC13B, DEC-205, GP200-MR6, C-type lectin domain family 13 member B)
Product Sizes
200ul
£495.00
364030-200UL
About this Product
- SKU:
- 364030
- Application:
- Western Blot, Immunohistochemistry
- Clonality:
- Polyclonal
- Extra Details:
- The LY75 protein has 77% overall aa identity with mouse Dec205. LY75 is a member of the MMR family of multidomain molecules. It contains a signal peptide, a cysteine-rich domain, a fibronectin type II domain, 10 carbohydrate recognition-like domains, a transmembrane domain, and a cytoplasmic tail. Northern blot analysis of hematopoietic cell lines revealed the presence of a predominant 7.8kb and a secondary 9.5kb transcript in myeloid, B-cell, and Hodgkin cell lines but not in the Jurkat T-cell line. In peripheral blood, immature DC contained barely detectable transcripts, which increased 50-fold upon differentiation and activation. LY75 acts as an endocytic receptor to direct captured antigens from the extracellular space to a specialized antigen-processing compartment. Applications: Suitable for use in ELISA, Western Blot, Immunohistochemistry, Immunocytochemistry. Other applications not tested. Recommended Dilution: ELISA: 1:100-5000 Western Blot: 1:50-400 Immunohistochemistry (frozen): 1:50-500 Immunohistochemistry (paraffin): 1:10-100 Immunocytochemistry: 1:50-500 Optimal dilutions to be determined by the researcher. AA Sequence: MGSSHHHHHH SSGLVPRGSH MASMTGGQQM GRGSEF- CEGNW EKNEQIGSCY QFNNQEILSW KEAYVSCQNQ GADLLSIHSA AELAYITGKE DIARLVWLGL NQLYSARGWE WSDFRPLKFL NWDPGTPVAP VIGGSSCARM DTESGLWQSV SCESQQPYVC KKPLNNTLEL PDVWTYTDTH CHVGWLPNNG FCYLLANESS SWDAAHLKCK AFGADLISMH SLADVEVVVT KLHNGDVKKE IWTGLKNTNS PALFQWSDGT EVTLTYWNEN EPSVPFNKTP NCVSYLGKLG QWKVQSCEKK LRYVCKKKGE ITKDAESDKL CPP Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
- Host:
- Rabbit
- Immunogen:
- Recombinant protein corresponding to Cys216-Pro503 of mouse LY75 expressed in E. coli.
- Isotype:
- IgG
- Physical State:
- Supplied as a liquid in PBS, pH 7.4, 0.02% sodium azide, 50% glycerol.
- Purity:
- Purified by Protein A and peptide affinity chromatography.
- Shipping Conditions:
- Blue Ice
- Specificity:
- Recognizes mouse LY75.
- Source:
- mouse
- Storage Conditions:
- -20[o]C
- Supplier:
- United States Biological
- Type:
- Antibodies:Polyclonal Antibody
