IRS1 (Insulin Receptor Substrate 1, HIRS1)
Product Sizes
200ul
£505.00
363762-200UL
About this Product
- SKU:
- 363762
- Application:
- Western Blot, Immunofluorescence
- Clonality:
- Polyclonal
- Extra Details:
- Insulin receptor substrate 1 plays a key role in transmitting signals from the IGF-1 receptors to intracellular pathways PI3K / Akt and Erk MAP kinase pathways. Tyrosine phosphorylation of the insulin receptors or IGF-1 receptors, upon extracellular ligand binding, induces the cytoplasmic binding of IRS-1 to these receptors, through its SH2 domains. IRS-1 plays important biological function for both metabolic and mitogenic (growth promoting) pathways: mice deficent of IRS1 have only a mild diabetic phenotype, but a pronounced growth impairment, i.e. IRS-1 knockout mice only reach 50% of the weight of normal mice. IRS-1 may also play a role in cancer, as it has been shown that transgenic mice overexpressing IRS-1 develop breast cancer. Applications: Suitable for use in Western Blot, Immunofluorescence and Immunocytochemistry. Other applications not tested. Recommended Dilution: ImmunocytochemistryImmunofluorescence: 5-20ug/ml Western Blot: 0.01-2ug/ml Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
- Host:
- Rabbit
- Immunogen:
- Recombinant protein corresponding to aa837-1089 of mouse IRS1 expressed in E. coli.
- Isotype:
- IgG
- Physical State:
- Supplied as a liquid in PBS, pH 7.4, 0.02% sodium azide, 50% glycerol.
- Purity:
- Purified by Protein A and peptide affinity chromatography.
- Shipping Conditions:
- Blue Ice
- Specificity:
- Recognizes mouse IRS1. Species Crossreactivity: human, rat and bovine
- Source:
- mouse
- Storage Conditions:
- -20[o]C
- Supplier:
- United States Biological
- Type:
- Antibodies:Polyclonal Antibody
