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Antibodies

IRF3 (Interferon Regulatory Factor 3)

Product Sizes
200ul
£495.00
363754-200UL
About this Product
SKU:
363754
Application:
Western Blot, Immunohistochemistry
Clonality:
Polyclonal
Extra Details:
Interferon regulatory factor 3 is a member of the interferon regulatory transcription factor (IRF) family. IRF3 is found in an inactive cytoplasmic form that upon serine/threonine phosphorylation forms a complex with CREBBP. This complex translocates to the nucleus and activates the transcription of interferons alpha and beta, as well as other interferon-induced genes. The IRF3 gene is present in a single copy in the human genome. Northern blot analysis detected a 1.6kb constitutively expressed IRF3 transcript. The deduced 427aa IRF3 protein is 34 to 40% identical to other members of the IRF family in the N-terminal region. Au et al. (1995) showed that IRF3 is a 50kD protein. Applications: Suitable for use in ELISA, Western Blot, Immunohistochemistry and Immunocytochemistry. Other applications not tested. Recommended Dilutions: Western Blot: 0.01-2ug/ml Immunohistochemistry (Paraffin): 5-20ug/ml Immunocytochemistry: 5-20ug/ml Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Host:
Rabbit
Immunogen:
Recombinant protein corresponding to aa1-406 of mouse IRF3 expressed in E. coli.
Isotype:
IgG
Physical State:
Supplied as a liquid in PBS, pH 7.4, 0.05% Proclin-300, 50% glycerol.
Purity:
Purified by Protein A and peptide affinity chromatography.
Shipping Conditions:
Blue Ice
Specificity:
Recognizes mouse IRF3. Species Crossreactivity: human and rat
Source:
mouse
Storage Conditions:
-20[o]C
Supplier:
United States Biological
Type:
Antibodies:Polyclonal Antibody