FNDC5 (Fibronectin Type III Domain Containing Protein 5, FRCP2, Irisin, Fibronectin type III repeat-containing protein 2)
Product Sizes
200ul
£550.00
363143-200UL
About this Product
- SKU:
- 363143
- Application:
- Immunohistochemistry
- Clonality:
- Polyclonal
- Extra Details:
- FNDC5 contains an N-terminal signal peptide, a fibronectin type III domain, and a transmembrane domain. The 2 proteins share 60% identity in the FNIII domain and 86% in the transmembrane domain. Northern blot analysis of multiple mouse tissues detected highest expression of Fndc5 in adult brain and heart, and low expression in lung, skeletal muscle, and testis. Northern blot analysis of mouse embryonic mRNA first detected Fndc5 expression at embryonic day 11, with strongest expression at E15 and decreasing expression at E17. In situ hybridization demonstrated expression of Fndc5 in the fore- and midbrain, as well as in the brachial arches and the heart. Overexpression of a GFP-Fndc5 fusion protein in COS cells showed localization predominantly in the endoplasmic reticulum, with small amounts in the plasma membrane. Applications: Suitable for use in Immunohistochemistry. Other applications not tested. Recommended Dilution: Immunohistochemistry: 5-20ug/ml Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
- Host:
- Rabbit
- Immunogen:
- Recombinant protein corresponding to aa77-144 of human FNDC5, expressed in E. coli.
- Isotype:
- IgG
- Physical State:
- Supplied as a liquid in PBS, pH 7.4, 0.05% Proclin-300, 50% glycerol.
- Purity:
- Purified by Protein A and peptide affinity chromatography.
- Shipping Conditions:
- Blue Ice
- Specificity:
- Recognizes human FNDC5. Species Crossreactivity: mouse, rat and porcine
- Source:
- human
- Storage Conditions:
- -20[o]C
- Supplier:
- United States Biological
- Type:
- Antibodies:Polyclonal Antibody
