VISTA, CT (V-Domain Ig Suppressor of T Cell Activation, Gi24) (PE)
Product Sizes
100ul
£748.00
351951-100UL
About this Product
- SKU:
- 351951
- Application:
- Flow Cytometry
- translate.label.attr.clone:
- D1L2G
- Clonality:
- Monoclonal
- Extra Details:
- VISTA (V-Domain Ig Suppressor of T Cell Activation) is a negative checkpoint control protein that regulates T cell activation and immune responses. VISTA, which contains a single Ig-like V-type domain, a transmembrane domain, and an intracellular domain, has sequence similarity to both the B7 and CD28 family members. Although primarily expressed by myeloid cells, VISTA is also expressed by CD4+, CD8+, and FoxP3+ T-cells. Thus, VISTA is described as both a ligand and a receptor. Blocking VISTA induces T-cell activation and proliferation, and potentiates disease severity in the EAE model. Furthermore, genetic deletion of VISTA in mice leads to spontaneous T-cell activation and chronic inflammation. In mouse models of cancer, neutralization of VISTA enhances T-cell proliferation and effector function and increases tumor infiltration, suggesting VISTA blockade could be an effective strategy for tumor immunotherapy. Applications: Suitable for use in Flow Cytometry. Other applications not tested. Recommended Dilutions: Flow Cytometry: 1:50 Optimal dilutions to be determined by the researcher. Storage and Stability: Store product at 4°C in the dark. DO NOT FREEZE! Stable at 4°C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: PE conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap.
- Host:
- Rabbit
- Immunogen:
- Synthetic peptide corresponding to residues near the carboxy terminal of human VISTA. protein
- Isotype:
- IgG
- Physical State:
- Supplied as a liquid in PBS, pH 7.2, 2mg/ml BSA, 0.09% sodium azide. Labeled with R-Phycoerythrin (PE).
- Purity:
- Purified
- Shipping Conditions:
- Blue Ice
- Specificity:
- Recognizes endogenous levels of total human VISTA. Species Crossreactivity: monkey
- Source:
- human
- Storage Conditions:
- 4[o]C Do Not Freeze
- Supplier:
- United States Biological
- Type:
- Antibodies:Monoclonal Antibody
