Cytochrome P450 2D6, Recombinant, Human (CYP2D6, LKM1, Liver/Kidney Microsomal Antigen 1)
Product Sizes
20ug
£927.00
208822-20UG
About this Product
- SKU:
- 208822
- Application:
- Western Blot
- Extra Details:
- Cytochrome P450 2D6 is a member of a complex family of microsomal enzymes (mono-oxygenases) present in the endoplasmic reticulum membrane, which perform detoxification reaction on xenobiotic compounds. Cytochrome P450 2D6 is the molecular target of autoantibodies against the "liver kidney microsomal antigen 1" (LKM 1) which has been classically defined by immunofluorescence microscopy. The presence of these autoantibodies is considered indicative of Autoimmune Hepatitis Type 2; LKM 1 antibodies have also been detected in patients with hepatitis C viral infection. The International Autoimmune Hepatitis Group therefore has subdivided the AIH type 2 into two subgroups: 2a with HCV and 2b without HCV. AIH 2a patients are often over 40 and predominantly male. The use of a purified recombinant Cytochrome P450 2D6 antigen allows the differentiation of autoimmune hepatitis from drug-induced hepatitis where transient autoantibodies to other P450 family members occur which cannot be differentiated by immunofluorescence techniques. Recombinant protein corresponding to human Cytochrome P450 2D6, expressed in sf9 cells. Swiss/Uniprot: P10635 Molecular Weight: ~55.8kD Applications: 1. Binds IgG-type human auto-antibodies. 2. Standard ELISA test (checker-board analysis of positive/negative sera panels, including international reference serum NIBSC 66/387). (coating concentration 0.35-0.6ug/ml depending on the type of ELISA plate. 3. Western-Blot with monoclonal anti-LKM-1 antibody and autoimmune hepatitis patient sera. Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing.. Store at -20°C. Aliquots are stable for 6 months after receipt at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
- Molecular Weight:
- 55.8
- Physical State:
- Supplied as a liquid in 50mM potassium phosphate, pH 7.5, 1mM DTT, 1% Triton X-100, 15% glycerol.
- Purity:
- ≥40% (SDS-PAGE)
- Shipping Conditions:
- Blue Ice
- Storage Conditions:
- -20[o]C
- Type:
- Biomaterials:Tissues
