GDNF, Recombinant, Human, Animal Free (Glial-Derived Neurotrophic Factor, ATF-1)
Product Sizes
2ug
£351.00
208624-2UG
About this Product
- SKU:
- 208624
- Extra Details:
- GDNF is a disulfide-linked homodimeric neurotrophic factor structurally related to Artemin, Neurturin and Persephin. These proteins belong to the cysteine-knot superfamily of growth factors that assume stable dimeric protein structures. GDNF signals through a multicomponent receptor system, composed of a RET and one of the four GFRalpha (alpha1-alpha4) receptors. GDNF specifically promotes dopamine uptake and survival and morphological differentiation of midbrain neurons. Using Parkinson's disease mouse model, GDNF has been shown to improve conditions such as bradykinesia, rigidity, and postural instability. The functional human GDNF ligand is a disulfide-linked homodimer, of two 15kD polypeptide chains called monomers. Each monomer contains seven conserved cysteine residues, one of which (Cys101) is used for inter-chain disulfide bridging and the others are involved in intramolecular ring formation known as the cysteine knot configuration. Manufactured using all Animal-Free reagents. Recombinant protein corresponding to human GDNF expressed in E. coli. Biological Activity: Determined by a cell proliferation assay using SH-SY5Y cells. The expected ED50 for this effect is 1.0-10ng/ml. Amino Acid Sequence: MSPDKQMAVL PRRERNRQAA AANPENSRGK GRRGQRGKNR GCVLTAIHLN VTDLGLGYET KEELIFRYCS GSCDAAETTY DKILKNLSRN RRLVSDKVGQ ACCRPIAFDD DLSFLDDNLV YHILRKHSAK RCGCI Storage and Stability: Lyophilized and reconstituted products are stable for 6 months after receipt at -20°C. Reconstitute with sterile ddH2O. Aliquot to avoid repeated freezing and thawing. Store at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
- Molecular Weight:
- 30.1
- Physical State:
- Supplied as a lyophilized powder from 10mM sodium citrate, 150mM sodium chloride. Reconstitute in sterile dH2O with a carrier protein to a concentration of 0.1-1mg/ml
- Purity:
- ≥98% (SDS-PAGE and HPLC). Endotoxin: ≤ 0.1ng/ug (1EU/ug)
- Shipping Conditions:
- Ambient
- Storage Conditions:
- -20[o]C
- Type:
- Proteins, Peptides, Small Molecules & Other Biomolecules:Recombinant Proteins
