NFkB, p65 (Nuclear Factor NF-kappaB p65 Subunit, Transcription Factor p65, RELA)
Product Sizes
100ul
£453.00
207301-100UL
About this Product
- SKU:
- 207301
- Application:
- Immunoprecipitation, Western Blot
- Clonality:
- Polyclonal
- Extra Details:
- NF-kB is a pleiotropic transcription factor present in almost all cell types and is the endpoint of a series of signal transduction events that are initiated by a vast array of stimuli related to many biological processes such as inflammation, immunity, differentiation, cell growth, tumorigenesis and apoptosis. NF-kB is a homo- or heterodimeric complex formed by the Rel-like domain-containing proteins RELA/p65, RELB, NFKB1/p105, NFKB1/p50, REL and NFKB2/p52. The heterodimeric p65-p50 complex is the most abundant complex. The dimers bind at k-B sites in the DNA of their target genes and the individual dimers have distinct preferences for different kB sites that they can bind with distinguishable affinity and specificity. Different dimer combinations act as transcriptional activators or repressors, respectively. NF-kB complexes are held in the cytoplasm in an inactive state complexed with members of the NF-kB inhibitor (I-kB) family. In a conventional activation pathway, I-kB is phosphorylated by I-kB kinases (IKKs) in response to different activators, subsequently degraded thus liberating the active NF-kB complex which translocates to the nucleus. NF-kB heterodimeric p65-p50 and p65-c-Rel complexes are transcriptional activators. The NF-kB p65-p65 complex appears to be involved in invasin-mediated activation of IL-8 expression. p65 shows a weak DNA-binding site which could contribute directly to DNA binding in the NF-kB complex. Applications: Suitable for use in Western Blot, Immunoprecipitation and Immunocytochemistry. Other applications not tested. Recommended Dilution: Western Blot: 1:2000-1:5000 Immunocytochemistry: 1:250-1:300 Immunoprecipitation: 2ug Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
- Host:
- Rabbit
- Immunogen:
- Recombinant protein corresponding to human NF-kB (p65 subunit).
- Isotype:
- IgG
- Physical State:
- Supplied as a liquid in 0.09% sodium azide.
- Purity:
- Purified
- Shipping Conditions:
- Blue Ice
- Specificity:
- Recognizes human NF-kB (p65 subunit). Species Crossreactivity: mouse
- Source:
- human
- Storage Conditions:
- -20[o]C
- Supplier:
- United States Biological
- Type:
- Antibodies:Polyclonal Antibody
