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Assay Kits & ELISAs

PDE4D3 BioAssay™ Kit

Product Sizes
1Kit
£1068.00
171971-1KIT
About this Product
SKU:
171971
Extra Details:
The PDE4D3 Assay Kit comes in a convenient 96-well format, with purified PDE4D3 enzyme, fluorescently labeled PDE4D substrate (cAMP), binding agent, and PDE assay buffer for 100 enzyme reactions. The key to the PDE4D3 BioAssay™ Kit is the specific binding agent. Using this kit, only two simple steps on a microtiter plate are required for PDE4D3 reactions. First, the fluorescently labeled cAMP is incubated with a sample containing PDE4D3 for 1 hour. Second, a binding agent is added to the reaction mix to produce a change in fluorescent polarization that can then be measured using a fluorescence reader equipped for the measurement of fluorescence polarization. Applications: Great for studying enzyme kinetics and screening small molecular inhibitors for drug discovery and HTS applications. Components: PDE4D3 recombinant enzyme, 1x1ug FAM-cyclic-3’, 5’-AMP (20um), 1x50ul PDE assay buffer, 1x25ml Binding Agent, 1x100ul Binding Agent Diluent, 1x10ml Black, low binding NUNC microtiter plate (VWR 62408-936), 1 plate Storage and Stability: Store powder at 4°C liquid at -20°C. Store other components at 4°C. Stable for at least 6 months For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Shipping Conditions:
Blue Ice
Storage Conditions:
-20[o]C/-70[o]C
Type:
Assay Kits:Assays