TTF-1 (BCH, Benign chorea, BHC, Homeobox protein Nkx2.1, NK2 homeobox 1, NKX2.1, NKX2A, TEBP, Thyroid nuclear factor-1, Thyroid specific enhancer binding protein, Thyroid transcription factor-1 (TTF-1), Tin man, TITF1, TTF-1) (BSA & Azide Free) (MaxLight 550)
Product Sizes
100ul
£876.00
168884-AF-ML550-100UL
About this Product
- SKU:
- 168884-AF-ML550
- Application:
- FLISA
- translate.label.attr.clone:
- 8G7G3/1
- Clonality:
- Monoclonal
- Extra Details:
- MaxLight™550 is a new Yellow-Green photostable dye conjugate comparable to Alexa Fluor™546, 555, DyLight™549 , Cy3™, TRITC and offers better labeling efficiency, brighter imaging and increased immunodetection. Absorbance (550nm); Emission (575nm); Extinction Coefficient 150,000. Recognizes a protein of 40kDa, identified as Thyroid transcription factor-1 (TTF-1). TTF-1 is a member of the NKx2 family of homeodomain transcription factors. It is expressed in epithelial cells of the thyroid gland and the lung. Nuclei from liver, stomach, pancreas, small intestine, colon, kidney, breast, skin, testes, pituitary, prostate, and adrenal glands are unreactive. Anti-TTF-1 is useful in differentiating primary adenocarcinoma of the lung from metastatic carcinomas originating in the breast, mediastinal germ cell tumors, and malignant mesothelioma. It can also be used to differentiate small cell lung carcinoma from lymphoid infiltrates. Loss of TTF-1 expression in non-small cell lung carcinoma has been associated with aggressive behavior of such neoplasms. TTF-1 reactivity is also seen in thyroid malignancies. Applications: Suitable for use in Immunofluorescence, FLISA, Flow Cytometry, Western Blot, Immunoprecipitation, Immunohistochemistry. Other applications not tested. Recommended Dilution: Flow Cytometry: 0.5-1ug/million cells Immunofluorescence: 0.5-1ug/ml Western Blot: 0.5-1.0ug/ml Immunoprecipitation: 1-2ug/500ug protein lysate Immunohistochemistry: Frozen & Formalin-fixed: 0.5-1ug/ml for 30 minutes at RT (Staining of formalin-fixed tissues requires boiling tissue sections in 10mM citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes Optimal dilutions to be determined by the researcher. Positive Control: MAD109, MLE-15, H441-4, or H345 cells. Normal thyroid or lung. Storage and Stability: Store product at 4°C in the dark. DO NOT FREEZE! Stable at 4°C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: MaxLight™550 conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap. Note: Applications are based on unconjugated antibody.
- Host:
- Mouse
- Immunogen:
- Rat full length TTF-1 recombinant protein. Cellular Localization: Nuclear
- Isotype:
- IgG1,k
- Physical State:
- Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with MaxLight™550.
- Purity:
- Purified by Protein A/G affinity chromatography.
- Shipping Conditions:
- Blue Ice
- Specificity:
- Recognizes human TTF-1. Species Crossreactivity: mouse, and rat. Shows a broad species reactivity.
- Source:
- human
- Storage Conditions:
- 4[o]C Do Not Freeze
- Type:
- Antibodies:Sera
