c-Myb Oncoprotein (c-myb_CDS, efg, ONCOGENE AMV, Oncogene MYB, Proto-oncogene c-Myb, Transcriptional activator Myb, v-myb avian myeloblastosis viral oncogene homolog) (BSA & Azide Free) (MaxLight 650)
Product Sizes
100ul
£876.00
168654-AF-ML650-100UL
About this Product
- SKU:
- 168654-AF-ML650
- Application:
- FLISA
- translate.label.attr.clone:
- MYB286
- Clonality:
- Monoclonal
- Extra Details:
- MaxLight™650 is a new Far-IR stable dye conjugate comparable to Alexa Fluor™647, DyLight™649, Cy5™ and offers better labeling efficiency, brighter imaging and increased immunodetection. Absorbance (655nm); Emission (676nm); Extinction Coefficient 250,000. The highly leukemogenic avian retrovirus E26 contains two oncogenes, v-Myb and v-Ets, which are expressed together as a fusion protein. The cellular hom- olog of v-Myb, designated c-Myb, encodes a transcription factor. Deletion or disruption of a negative regulatory domain mapping within the carboxy-ter- minal domain of c-Myb results in enhanced transactivating capacity and in parallel, leads to activation of its ability to transform hemopoietic cells. c-Myb is expressed preferentially, but not exclusively, in immature hemopoietic cells and its expression decreases as cells differentiate. Applications: Suitable for use in Immunofluorescence, Flow Cytometry, FLISA, Western Blot, Immunoprecipitation. Other applications not tested. Recommended Dilution: Flow Cytometry: 0.5-1ug/million cells Immunofluorescence: 1-2ug/ml Western Blot: 0.5-1ug/ml Immunoprecipitation: 1-2ug/500ug protein lysate Optimal dilution for a specific application should be determined Positive Control: Jurkat, MOLT-4, COLO or BT-20 cells. Colon or breast carcinomas. Storage and Stability: Store product at 4°C in the dark. DO NOT FREEZE! Stable at 4°C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: MaxLight™650 conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap. Note: Applications are based on unconjugated antibody.
- Host:
- Mouse
- Immunogen:
- A synthetic peptide, corresponding to aa 119-135 (RRKVEQEGYPQESSKAG) of c-myb oncoprotein, coupled to KLH. Cellular Localization: Nuclear
- Isotype:
- IgG1,k
- Physical State:
- Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with MaxLight™650.
- Purity:
- Purified by Protein A/G affinity chromatography.
- Shipping Conditions:
- Blue Ice
- Specificity:
- Recognizes human c-Myb Oncoprotein.
- Source:
- human
- Storage Conditions:
- 4[o]C Do Not Freeze
- Type:
- Antibodies:Sera
