a-Fetoprotein (Alpha fetoglobulin, FETA, HPAFP) (BSA & Azide Free) (MaxLight 650)
Product Sizes
100ul
£876.00
168643-AF-ML650-100UL
About this Product
- SKU:
- 168643-AF-ML650
- Application:
- Immunofluorescence, Immunohistochemistry
- translate.label.attr.clone:
- C3
- Clonality:
- Monoclonal
- Extra Details:
- MaxLight™650 is a new Far-IR stable dye conjugate comparable to Alexa Fluor™647, DyLight™649, Cy5™ and offers better labeling efficiency, brighter imaging and increased immunodetection. Absorbance (655nm); Emission (676nm); Extinction Coefficient 250,000. It recognizes an oncofetal glycoprotein with a single chain of 70kDa, which is identified as alpha fetoprotein (AFP). This MAb is highly specific to AFP and shows no cross-reaction with other oncofetal antigens or serum albumin. AFP is normally synthesized in the liver, intestinal tract, and yolk sac of the fetus. Antibody to AFP has been shown to be useful in detecting hepatocellular carcinomas (HCC) and germ cell neoplasms, especially yolk sac tumors. Applications: Suitable for use in Immunofluorescence and Immunohistochemistry. Other applications not tested. Recommended Dilution: Immunohistochemistry (formalin-fixed): Requires HIER using 10mM sodium citrate buffer pH 6.0 for 10-20min followed by cooling at RT for 20min Optimal dilutions to be determined by the researcher. Positive Control: Hep-G2 cells. Fetal liver or hepatocellular carcinoma Storage and Stability: Store product at 4°C in the dark. DO NOT FREEZE! Stable at 4°C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: MaxLight™650 conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap. Note: Applications are based on unconjugated antibody.
- Host:
- Mouse
- Immunogen:
- alpha-Fetoprotein purified from serum of a hepatoma patient. Cellular Localization: Cytoplasmic
- Isotype:
- IgG2a,k
- Physical State:
- Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with MaxLight™650.
- Purity:
- Purified by Protein A/G affinity chromatography.
- Shipping Conditions:
- Blue Ice
- Specificity:
- Recognizes human alpha-Fetoprotein. Species Crossreactivity: monkey, canine, and porcine. It does not react with bovine, mouse, and rat.
- Source:
- human
- Storage Conditions:
- 4[o]C Do Not Freeze
- Type:
- Antibodies:Sera
