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Proteins and Peptides

N-Terminal Pro-Brain Natriuretic Peptide (NT-ProBNP) Recombinant, Human

Product Sizes
10ug
£372.00
156033-10UG
About this Product
SKU:
156033
Extra Details:
Recombinant protein corresponding to aa27-102 with an N-Terminal His-Tag from human NT-ProBNP, expressed in E. coli. Uniprot/Accession: P16860 Predicted Isoelectric Point: 6.3 Predicted Molecular Weight: 12.2kD Accurate Molecular Weight: 12kD as determined by SDS-PAGE reducing conditions Subcellular Location: Secreted Amino Acid Sequence: HPLG SPGSASDLET SGLQEQRNHL QGKLSELQVE QTSLEPLQES PRPTGVWKSR EVATEGIRGH RKMVLYTLRA PR Applications: Suitable for use in ELISA, Western Blot and SDS-PAGE. Other applications not tested. Recommended Dilutions: Optimal dilutions to be determined by the researcher. Storage and Stability: Lyophilized and reconstituted products are stable for 6 months after receipt at -70°C. Reconstitute with 20mM Tris, 150mM sodium chloride, pH 8.0 . Aliquot to avoid repeated freezing and thawing. Store at -70°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer. Note: Thermal stability is described by the loss rate of the target protein. The loss rate was determined by accelerated thermal degradation by incubating the protein at 37°C for 48h, with no obvious degradation or precipitation observed. Protein loss is <5% within the expiration date under appropriate storage conditions.
Molecular Weight:
12
Physical State:
Supplied as a lyophilized powder from 20mM Tris, 150mM sodium chloride, pH 8.0, 1mM EDTA, 0.0.1% sarcosyl, 5% trehalose, 0.05% Procln-300. Reconstitute with 20mM Tris, 150mM sodium chloride, pH 8.0 t
Purity:
≥95%
Shipping Conditions:
Blue Ice
Storage Conditions:
-70[o]C
Supplier:
United States Biological
Type:
Proteins, Peptides, Small Molecules & Other Biomolecules:Recombinant Proteins