Interleukin 10 (IL10) Recombinant, Mouse
Product Sizes
10ug
£412.00
155342-10UG
About this Product
- SKU:
- 155342
- Extra Details:
- Recombinant protein corresponding to aa19-178 with two N-terminal tags, His-tag and T7-tag from mouse expressed in E. coli Fragment: Ser19~Ser178 (P18893) Amino Acid Sequence: MGSSHHHHHHSSGLVPRGSHMASMTGGQQMGRGS-SR GQYSREDNNC THFPVGQSHM LLELRTAFSQ VKTFFQTKDQ LDNILLTDSL MQDFKGYLGC QALSEMIQFY LVEVMPQAEK HGPEIKEHLN SLGEKLKTLR MRLRRCHRFL KCENKSKAVE QVKSDFNKLE DQGVYKAMNE FDIFINCIEA YMMIKMKS Epitope Tag: Two N-terminal tags, His-tag and T7-tag Predicted Molecular Weight: 22.3kD Subcellular Location: Secreted Predicted isoelectric point: 8.8 Applications: Suitable for use in ELISA, Western Blot, Immunoprecipitation and SDS-PAGE. Other applications not tested. Recommended Dilutions: Optimal dilutions to be determined by the researcher. Included Protein Molecular Weight Marker: 168631: Protein Molecular Weight Marker, Unstained Ready to use, No heating, diluting or reducing agents needed. MW: 10kD, 14kD, 18kD, 22kD, 26kD, 33kD, 44kD, 70kD Double intensity: 10kD, 18kD, 26kD Supplied in 62.5mM Tris-H3PO4, pH 7.5, 1mM EDTA, 2% SDS, 100mM DTT, 1mM sodium azide, 0.01% bromo-phenol blue, 33% glycerol. Add 3-5ul/well for mini gels; 7ul/well for large gels. Store at -20°C Stable for 6 months after receipt. Storage and Stability Lyophilized and reconstituted products are stable for 6 months after receipt at -70°C. Reconstitute with sterile 20mM Tris, 150mM sodium chloride, pH 8.0.. Aliquot to avoid repeated freezing and thawing. Store at -70°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer. Note: Thermal stability is described by the loss rate of the target protein. The loss rate was determined by accelerated thermal degradation by incubating the protein at 37°C for 48h, with no obvious degradation or precipitation observed. Protein loss is <5% within the expiration date under appropriate storage conditions.
- Molecular Weight:
- 22.3
- Physical State:
- Supplied as a lyophilized powder in 20mM Tris, 150mM sodium chloride, pH 8.0, 1mM EDTA, 1mM DTT, 0.01% SKL, 5% Trehalose, Proclin300. Reconstitute with sterile 20mM Tris, 150mM sodium chloride, pH 8.0
- Purity:
- ~95%. Endotoxin: ≤1.0EU/ug(LAL)
- Shipping Conditions:
- Blue Ice
- Storage Conditions:
- -20[o]C
- Type:
- Proteins, Peptides, Small Molecules & Other Biomolecules:Recombinant Proteins
