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Apolipoprotein E (APOE) Recombinant, Mouse

Product Sizes
10ug
£412.00
153583-10UG
About this Product
SKU:
153583
Extra Details:
Recombinant protein corresponding to aa14-167 with an N-Terminal His Tag and a GST-Tag from mouse APOE expressed in E. coli. Swiss/UniProt Accession: P08226 Amino Acid Sequence: TGCLAEG EPEVTDQLEW QSNQPWEQAL NRFWDYLRWV QTLSDQVQEE LQSSQVTQEL TALMEDTMTE VKAYKKELEE QLGPVAEETR ARLGKEVQAA QARLGADMED LRNRLGQYRN EVHTMLGQST EEIRARLSTH LRKMRKRLMR DAEDLQK Predicted Isoelectric Point: 5.7 Predicted Molecular Weight: 48kD Accurate Molecular Weight: 48kD as determined by SDS-PAGE reducing conditions Subcellular Localization: Secreted Applications: Suitable for use in ELISA, Western Blot and SDS-PAGE. Other applications not tested. Recommended Dilutions: Optimal dilutions to be determined by the researcher. Storage and Stability: Lyophilized powder may be stored at -70°C. Stable for 6 months after receipt. Reconstitute with 20mM Tris, 150mM sodium chloride, pH 8.0 . Aliquot to avoid repeated freezing and thawing. Store at -70°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer. Note: Thermal stability is described by the loss rate of the target protein. The loss rate was determined by accelerated thermal degradation by incubating the protein at 37°C for 48h, with no obvious degradation or precipitation observed. Protein loss is <5% within the expiration date under appropriate storage conditions.
Molecular Weight:
48
Physical State:
Supplied as lyophilized powder from 20mM Tris, 150mM sodium chloride, pH 8.0, 1mM EDTA, 1mM DTT, 0.01% sarcosyl, 5% trehalose, 0.05% Proclin. Reconstitute 20mM Tris, 150mM sodium chloride, pH 8.0 to a
Purity:
≥95%
Shipping Conditions:
Blue Ice
Storage Conditions:
-70[o]C
Supplier:
United States Biological
Type:
Proteins, Peptides, Small Molecules & Other Biomolecules