Alpha-2-Heremans Schmid Glycoprotein (aHSG, Alpha-2-HS-Glycoprotein, A2HS, AHS, FETUA) Recombinant, Mouse
Product Sizes
10ug
£412.00
153449-10UG
About this Product
- SKU:
- 153449
- Extra Details:
- AHSG, also known as fetuin-A, belongs to the fetuin class of plasma binding proteins. It is involved in several functions, such as endocytosis, brain development and the formation of bone tissue. It is commonly present in the cortical plate of the immature cerebral cortex and bone marrow hemopoietic matrix, and it has been postulated that it participates in the development of the tissues. It forms soluble complexes with calcium and phosphate and thus is a carrier of otherwise insoluble calcium phosphate. Alpha2-HS glycoprotein, a glycoprotein present in the serum, is synthesized by hepatocytes. The AHSG molecule consists of two polypeptide chains, which are both cleaved from a proprotein encoded from a single mRNA. It is involved in several functions, such as endocytosis, brain development and the formation of bone tissue. The protein is commonly present in the cortical plate of the immature cerebral cortex and bone marrow hemopoietic matrix, and it has therefore been postulated that it participates in the development of the tissues. However, its exact significance is still obscure.[3] The choroid plexus is an established extrahepatic expression site. The mature circulating AHSG molecule consists of two polypeptide chains, which are both cleaved from a proprotein encoded from a single mRNA. Multiple post-translational modifications have been reported.[4] Thus AHSG is a secreted partially phosphorylated glycoprotein with complex proeolytic processing that circulates in blood and extracellular fluids. In the test tube AHSG can bind multiple ligands and therefore has been claimed to be involved in several functions, such as endocytosis, brain development and the formation of bone tissue. Most of these functions await confirmation in vivo. Fetuins like histidine-rich glycoprotein and kininogens are secreted (so-called type 3) cystatins.[5] Fetuins are carrier proteins like albumin. Fetuin-A forms soluble complexes with calcium and phosphate and thus is a carrier of otherwise insoluble calcium phosphate.[6][7] Thus fetuin-A is a potent inhibitor of pathological calcification. Mice deficient in fetuin-A show systemic calcification of soft tissues.[8][9] Source: Recombinant Mouse from E. coli Accession No: P29699 Fragment: Ala19~Phe250 (Accession No: P29699) Sequence: MGHHHHHHSGSEF-AP QGTGLGFREL ACDDPEAEQV ALLAVDYLNN HLLQGFKQVL NQIDKVKVWS RRPFGVVYEM EVDTLETTCH ALDPTPLANC SVRQLTEHAV EGDCDFHILK QDGQFRVMHT QCHSTPDSAE DVRKLCPRCP LLTPFNDTNV VHTVNTALAA FNTQNNGTYF KLVEISRAQN VPLPVSTLVE FVIAATDCTA KEVTDPAKCN LLAEKQHGFC KANLMHNLGG EEVSVACKLF Epitope Tag: N-terminal His-Tag Molecular Weight: 27.1kD Applications: Suitable for use in ELISA, Western Blot and SDS-PAGE. Other applications not tested. Recommended Dilution: Optimal dilutions to be determined by the researcher. Storage and Stability: Lyophilized and reconstituted products are stable for 6 months after receipt at -70°C. Reconstitute with sterile PBS. Aliquot to avoid repeated freezing and thawing. Store at -70°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer. Note: Thermal stability is described by the loss rate of the target protein. The loss rate was determined by accelerated thermal degradation by incubating the protein at 37°C for 48h, with no obvious degradation or precipitation observed. Protein loss is <5% within the expiration date under appropriate storage conditions.
- Molecular Weight:
- 27.1
- Physical State:
- Supplied as lyophilized powder from PBS, pH 7.4, 1mM DTT, 5% trehalose, 0.01% sarcosyl, 0.05% Proclin-300. Reconstitute with sterile PBS to a concentration of 0.1-1mg/ml. Do not vortex.
- Purity:
- ≥95%
- Shipping Conditions:
- Blue Ice
- Storage Conditions:
- 4[o]C/-70[o]C
- Type:
- Proteins, Peptides, Small Molecules & Other Biomolecules
