Acid Sphingomyelinase (ASM) Recombinant, Human
Product Sizes
10ug
£369.00
153305-10UG
About this Product
- SKU:
- 153305
- Extra Details:
- Recombinant protein corresponding to aa321-581 with an N-Terminal His Tag from human ASM, expressed in E. coli. Enzyme Activity: Not Determined. This product is recommended for use in applications that do not require a catalytically active form of the protein. Amino Acid Sequence: HE STPVNSFPPP FIEGNHSSRW LYEAMAKAWE PWLPAEALRT LRIGGFYALS PYPGLRLISL NMNFCSRENF WLLINSTDPA GQLQWLVGEL QAAEDRGDKV HIIGHIPPGH CLKSWSSWNYY RIVARYENTL AAQFFGHTHV DEFEVFYDEE TLSRPLAVAF LAPSATTYIG LNPGYRVYQI DGNYSGSSHV VLDHETYILN LTQANIPGAI PHWQLLYRAR ETYGLPNTLP TAWHNLVYRM RGDMQLFQTF WFLYHKGHP Predicted Isoelectric Point: 6.6 Predicted Molecular Mass: 33.8kD Accurate Molecular Mass: 30kD as determined by SDS-PAGE reducing conditions Subcellular Location: Lysosome Applications: Suitable for use in ELISA, Western Blot and SDS-PAGE. Other applications not tested. Recommended Dilutions: Optimal dilutions to be determined by the researcher. Storage and Stability: Lyophilized and reconstituted products are stable for 6 months after receipt at -70°C. Reconstitute with sterile 20mM Tris, 150mM sodium chloride, pH 8.0.. Aliquot to avoid repeated freezing and thawing. Store at -70°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer. Note: Thermal stability is described by the loss rate of the target protein. The loss rate was determined by accelerated thermal degradation by incubating the protein at 37°C for 48h, with no obvious degradation or precipitation observed. Protein loss is <5% within the expiration date under appropriate storage conditions.
- Molecular Weight:
- 30
- Physical State:
- Supplied as lyophilized powder from 20mM Tris, 150mM sodium chloride, pH 8.0, 1mM EDTA, 1mM DTT, 5% trehalose, 0.01% sarcosyl, 0.05% Proclin-300. Reconstitute with sterile 20mM Tris, 150mM sodium chlo
- Purity:
- ≥95%
- Shipping Conditions:
- Blue Ice
- Storage Conditions:
- -70[o]C
- Supplier:
- United States Biological
- Type:
- Proteins, Peptides, Small Molecules & Other Biomolecules:Recombinant Proteins
