Integrin alpha 9 beta 1, Recombinant, Mouse (CD29)
Product Sizes
50ug
£876.00
145813-50UG
About this Product
- SKU:
- 145813
- Extra Details:
- Integrin alpha 9 beta 1 is one of twelve integrin family adhesion receptors that share the beta 1 (CD29) subunit (13). It is the noncovalent heterodimer of 150kD alpha 9 and 130kD beta 1 type I transmembrane glycoprotein subunits (3). The alpha 9 extracellular domain (ECD) contains an Nterminal betapropeller structure, followed by domains termed thigh, calf1 and calf2 (2). The beta 1 ECD contains a vWFA domain, which interacts with the alpha 9 betapropeller to form a binding domain when the dimer is in the active, extended and open conformation. The 1006 amino acid (aa) mouse alpha 9 extracellular domain (ECD) shares 95% and 89% aa sequence identity with rat and human alpha 9, respectively, while the 708 aa mouse beta 1 ECD shares 98% aa identity with rat and 9394% with human, bovine, porcine, ovine, canine and feline beta 1. Each subunit has a transmembrane sequence and a short cytoplasmic tail which, for alpha 9, contains binding sites for paxillin and SSAT (spermine/spermidineNacetyltransferase) (4, 5). Integrin alpha 9 beta 1 inhibits cell spreading via signaling through paxillin (4). SSAT interaction with alpha 9 beta 1 allows catalysis that lifts spermine/spermidine inhibition of KIR potassium channels, and is shown to enhance cell migration or suppress airway smooth muscle contraction (5, 6). Lymphangiogenic vascular endothelia growth factors VEGFC and VEGFD are ligands for Integrin alpha 9 beta 1 (7, 8) Genetic deletion of mouse alpha 9 beta 1 results in a fatal chylothorax due to abnormal lymphatic development (9). Integrin alpha 9 beta 1 interacts in cis with GCSF R and enhances its signals, contributing to granulopoiesis (10). It also binds VCAM1, which promotes neutrophil transendothelial migration and delays apoptosis (11, 12). Other ligands for alpha 9 beta 1 include polydom/SVEP1, tenascin, osteopontin, nerve growth factor, and most members of the ADAM family (1316). Source: Recombinant protein corresponding to mouse Integrin alpha 9 beta 1, CHO-derived. Molecular Weight: ~120-160kD Activity: Measured by the ability of the immobilized protein to support the adhesion of CHO Chinese hamster ovary cells transfected with VCAM-1. The ED50 for this effect is typically 0.6-3ug/ml. Storage and Stability: Lyophilized and reconstituted products are stable for 6 months after receipt at -20°C. Reconstitute with sterile PBS. Aliquot to avoid repeated freezing and thawing. Store at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
- Molecular Weight:
- 120160
- Physical State:
- Supplied as a lyophilized powder in PBS. Reconstitute with sterile PBS.
- Purity:
- ~95% (SDS–PAGE). Endotoxin: ≤0.10EU/1ug (LAL).
- Shipping Conditions:
- Blue Ice
- Storage Conditions:
- -20[o]C
- Supplier:
- United States Biological
- Type:
- Proteins, Peptides, Small Molecules & Other Biomolecules:Recombinant Proteins
