GGTA1, Recombinant, Mouse, aa80-394 (alpha3GalT, Alpha Gal, GALT, Glt6d1)
Product Sizes
10ug
£876.00
145795-10UG
About this Product
- SKU:
- 145795
- Extra Details:
- GGTA1, also designated as alpha3GalT, is an alpha1,3 galactosyltransferase that catalyzes the transfer of galactose from UDPalphaDgalactose to an alpha1,3linkage with nonreducing terminal alphagalactosyl moieties present in glycoconjugates (1). This enzyme is expressed in many mammalian species but is absent from humans, apes, and Old World monkeys due to the mutational inactivation of the gene (2). Therefore, humans do not have galactosyl alpha1,3galactosyl structures on glycoconjugates and produce large amounts of antibody to this alphaGal epitope. The production of this endogenous antibody results from exposure to intestinal bacteria and other antigens (3). GGTA1 is of medical interest because the immune responses may be enhanced when selected targets are decorated with galactosyl alpha1,3galactosyl structures (4). Additionally, the presence of antialphaGal antibodies is a barrier to xenotransplantation of organs, therefore, genetic manipulation of the GGTA1 gene and the development of inhibitors of the enzyme are of interest as complementary approaches to reduce rejection of xenotransplanted organs. Structurally and mechanistically GGTA1 is a model for several homologous glycosyltransferases that differ in donor and acceptor substrate specificity, including the histo blood group A and B glycosyltransferases, Forssman glycolipid synthase, and isogloboside 3 synthase (5). The activity of the recombinant GGTA1 is measured using a phosphatasecoupled glycosyltransferase assay (6). Source: Recombinant protein corresponding to aa80-394, Trp80-Val394, from mouse alpha3GalT/GGTA1, fused to 6-his tag at C-terminal, NS0-derived. Molecular Weight: ~35-43kD Endotoxin: <1.0EU/1ug (LAL method). Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
- Molecular Weight:
- 3543
- Physical State:
- Supplied as a liquid in Tris, NaCl, Brij–35. BSA free.
- Purity:
- ~95% (SDS-PAGE)
- Shipping Conditions:
- Blue Ice
- Storage Conditions:
- -20[o]C
- Supplier:
- United States Biological
- Type:
- Proteins, Peptides, Small Molecules & Other Biomolecules:Recombinant Proteins
